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Antibacterial and oral malodour supressing effects of black cumin food materials and elucidation of these mechanisms

Research Project

Project/Area Number 17K12032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Social dentistry
Research InstitutionTsurumi University

Principal Investigator

Ishikawa Masao  鶴見大学, 歯学部, 非常勤講師 (50597250)

Co-Investigator(Kenkyū-buntansha) 村田 貴俊  鶴見大学, 歯学部, 講師 (10313529)
泉福 英信  国立感染症研究所, 細菌第一部, 室長 (20250186)
花田 信弘  鶴見大学, 歯学部, 教授 (70180916)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsブラッククミン / チモキノン / 口臭 / メチルメルカプタン / Fusobacterium nucleatum / メチオニナーゼ / 殺菌作用 / 酵素阻害作用 / メチニナーゼ / 殺菌剤CPC / Fusobacterium nucleatum / メチオニン代謝活活性 / メチオニンγ-リアーゼ / バイオフィルム / 細菌 / 歯学 / 植物
Outline of Final Research Achievements

Objectives: Black cumin (Nigella sativa L.) seeds and its essential oil (BC) have been widely used in functional foods, nutraceuticals and pharmaceutical products. Recently BC had deodorizing activity to methyl mercaptan that is a main cause of oral malodor and produced by Fusobacterium nucleatum (Fn). To utilize BC as a preventive material of oral malodor, we examined the effect of antibacterial and metabolic activity of Fn by BC and their components.
Results: BC and thymoquinone (TQ) had antibacterial activity on Fn at 0.003%~0.006%. These compounds had inhibited methioninase at sub -MIC level (0.001%). Kinetic study of methioninase indicated Michaelis constant (Km) is 4.6mM and the inhibition of TQ showed as mixed non-competitive inhibition. From these results, BC or TQ had more than 3 effective mechanisms to decrease oral malodor by antibacterial effect, L-methionine metabolic inhibition and by methioninase inhibition on Fn. BC is a useful material for prevention of oral malodor.

Academic Significance and Societal Importance of the Research Achievements

口臭は、働き盛りのサラリーマンの7割が自覚しており(企業調査:2004)、口臭との関連性が高い歯周病の有病率も30代以上の男性で8割以上(厚労省調査:2014)との報告があり、国民の口臭に対する意識は高い。
口臭研究は、学術的に、う蝕、歯周病の研究と比べ報告数は少なく、ニーズの高い分野であるにもかかわらず、メカニズムに基づいた基礎研究や、実効性が期待できる天然成分についての研究は殆どない。さらに、口臭抑制成分に使われている多くは、合成殺菌剤やカチオン性界面活性剤であり、安全性面での問題点も指摘されている。その中、天然物の口臭抑制成分に対する関心は高まってきた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Effect of thymoquinone on Fusobacterium nucleatum-associated biofilm and inflammation2020

    • Author(s)
      Tada A, Nakayama-Imaohji H, Yamasaki H, Elahi M, Nagao T, Yagi H, Ishikawa M, Shibuya K, Kuwahara T
    • Journal Title

      Molecular Medicine Reports

      Volume: 22 Issue: 2 Pages: 643-650

    • DOI

      10.3892/mmr.2020.11136

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Purification of a High Molecular Mass Protein in Streptococcus mutans.2019

    • Author(s)
      Murata T , Yamashita M , Ishikawa M , Shibuya K , Hanada N
    • Journal Title

      Journal of visualized experiments : JoVE

      Volume: 151 Issue: 151 Pages: 151-151

    • DOI

      10.3791/59804

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 銅含有ガラス粉末のメチルメルカプタンに対する消臭効果2019

    • Author(s)
      渋谷耕司,石川綾子,花田信弘,石川正夫
    • Organizer
      日本歯科保存学会 2019年度春季学術大会(第150回)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Effect of Black Cumin Seed Oil on Methionine γ-lyase from Fusobacterium nucleatum2019

    • Author(s)
      Masao Ishikawa, Takatoshi Murata, Nobuhiro Hanada, Hidenobu Senpuku, Koji Shibuya
    • Organizer
      CED-IADR/NOF Oral Health Research Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ブラッククミンおよび殺菌剤のカンジダに対する抗真菌活性について2019

    • Author(s)
      石川正夫、村田貴俊、岡本公彰、泉福英信、花田信弘、渋谷耕司
    • Organizer
      第68回日本口腔衛生学会・総会
    • Related Report
      2018 Research-status Report
  • [Presentation] チモキノンのフソバクテリウム含有バイオフィルムに対する効果2018

    • Author(s)
      多田彩乃、石川正夫、渋谷耕司、桑原知巳
    • Organizer
      第38回日本歯科薬物療法学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] チモキノンのFusobacterium nucleatum混合バイオフィルムに対する効果2018

    • Author(s)
      多田彩乃、石川正夫、渋谷耕司、桑原知巳
    • Organizer
      第67回日本口腔衛生学会・総会
    • Related Report
      2017 Research-status Report
  • [Presentation] ブラッククミンおよび殺菌剤のFusobacterium nucleatumのメチルメルカプタン産生に及ぼす影響2018

    • Author(s)
      石川正夫、村田貴俊、泉福英信、花田信弘、渋谷耕司
    • Organizer
      第67回日本口腔衛生学会・総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Antibacterial Effects of Black Cumin Seed Components on Fusobacterium Biofilm2018

    • Author(s)
      Masao Ishikawa, Hidenobu Senpuku, Takatoshi Murata, Nobuhiro Hanada and Koji Shibuya
    • Organizer
      The 96th General Session of the IADR
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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