Reduction of waste seaweeds by a novel seaweed-degrading bacterium strain Myt-1 and development of sustainable recycling methods

• Project/Area Number: 17K12848
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Design and evaluation of sustainable and environmental conscious system
• Research Institution: University of Toyama
• Principal Investigator: SAKATOKU Akihiro 富山大学, 学術研究部理学系, 講師 (20713142)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 海藻分解菌 / バイオマス / 廃棄海藻 / 多糖分解酵素 / アルギン酸リアーゼ

Outline of Final Research Achievements

It is an important research subject to develop the sustainable and effective utilization of seaweed waste as "marine biomass". We isolated a novel Saccharophagus species (strain Myt-1), which can degrade several types of seaweed, from marine sediment of Toyama Bay.
In this study, the effectiveness of seaweed degradation products produced by Myt -1 strain was verified, and polysaccharides degrading enzymes were cloned from strain Myt-1, and the features were analyzed.

Academic Significance and Societal Importance of the Research Achievements

これまでにも海藻藻体そのものを分解することができる細菌の単離報告はあるが, 褐藻, 紅藻, 緑藻の3種全ての海藻藻体を分解できる細菌の報告はほとんどない。そのような中, 我々は, 富山湾の海底堆積物から3種全ての海藻藻体を分解できる細菌 (Myt-1株) の単離に成功した。
このMyt-1株が海藻を分解することによって産生したオリゴ糖には, 抗酸化活性が認められたことから, 廃棄海藻をマリンバイオマスとして捉えた有効利用に繋がる可能性が強く示唆された。さらに, Myt-1株が産生するアルギン酸リアーゼ (AlgMytE) は耐塩性と界面活性剤耐性が高く, 様々な産業への利用が期待された。

Research Products

• [Journal Article] Airborne Microbial Communities at High-Altitude and Suburban Sites in Toyama, Japan Suggest a New Perspective for Bioprospecting (2019)
  • Author(s): Daisuke Tanaka, Kei Sato, Motoshi Goto, So Fujiyoshi, Fumito Maruyama, Shunsuke Takato, Takamune Shimada, Akihiro Sakatoku, Kazuma Aoki, Shogo Nakamura
  • Journal Title: Frontiers in Bioengineering and Biotechnology Volume: 7 Pages: 12-12
  • DOI: 10.3389/fbioe.2019.00012
  • NAID: 120006782205
  • Peer Reviewed / Open Access
• [Journal Article] Newly isolated bacterium Tenacibaculum sp. strain Pbs-1 from diseased pearl oysters is associated with black-spot shell disease (2018)
  • Author(s): Akihiro Sakatoku, Takuya Fujimura, Michiko Ito, Seigo Takashima, Tadashi Isshiki
  • Journal Title: Aquaculture Volume: 493 Pages: 61-67
  • DOI: 10.1016/j.aquaculture.2018.04.049
  • Peer Reviewed
• [Journal Article] Discovery and biogeochemistry of asphalt seeps in the North Sao Paulo Plateau, Brazilian Margin (2018)
  • Author(s): Kai Jiang, Jing Zhang, Akihiro Sakatoku, Shota Kambayashi, Toshiro Yamanaka, Toshiyuki Kanehara, Katsunori Fujikura, Vivian Helena Pellizari
  • Journal Title: Scientific Reports Volume: 8 Issue: 1
  • DOI: 10.1038/s41598-018-30928-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Isolation and characterization of a novel seaweed-degrading bacterium (2019)
  • Author(s): Akihiro SAKATOKU
  • Organizer: A SPECIAL RESEARCH PRESENTATION (Universidad de La Frontera, Temuco, Chile)
  • Int'l Joint Research / Invited
• [Presentation] 新規海藻分解菌Zobellia sp. TY-1株の単離とキャラクタリゼーション (2018)
  • Author(s): 山下陽也, 田中大祐, 中村省吾, 酒徳昭宏
  • Organizer: 環境バイオテクノロジー学会2018年度大会
• [Presentation] 富山湾表層海水中の海藻分解菌の分布 (2017)
  • Author(s): 青山拓生, 田中大祐, 中村省吾, 酒徳昭宏
  • Organizer: 第19回マリンバイオテクノロジー学会仙台大会
• [Presentation] 海藻分解菌Saccharophagus sp. Myt-1株の海藻分解機構 (2017)
  • Author(s): 石川雅仁, 田中大祐, 中村省吾, 酒徳昭宏
  • Organizer: 第19回マリンバイオテクノロジー学会仙台大会
• [Presentation] 廃棄海藻の減容化とマリンバイオマスとして捉えた有効利用 (2017)
  • Author(s): 酒徳昭宏
  • Organizer: 平成29年度日本応用糖質科学会東日本支部ミニシンポジウム
  • Invited