Different Amino Acids Quality and Quantity and Metabolic Syndrome: Interaction between Bile Acids and Microbiota

• Project/Area Number: 17K12911
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Keio University
• Principal Investigator: YOKOYAMA Yoko 慶應義塾大学, 政策・メディア研究科(藤沢), 特任助教 (10617244)
• Research Collaborator: WATANABE Mitsuhiro ; FUKUDA Shinji ; TOMITA Masaru ; HONDA Akira
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 胆汁酸 / 栄養バランス / 腸内細菌 / 糖尿病 / 代謝疾患 / タンパク質

Outline of Final Research Achievements

The optimal balance of nutrient sources of energy has not been clarified. The meta-analysis did not confirm the metabolic disease improvement effect by higher protein diet. As a result of research on experimental animals, metabolic diseases were improved by lower protein diet. Bile acid composition, intestinal microbiota, metabolites, and microarray were performed to clarify the mechanism. In the lower protein group, brown adipose tissue function were improved. In the lower protein group, as a result of microbiota and metabolite analysis, anti-aging substance in cecal contents were increased. Differences and complex associations between 30 kinds of bile acids composition and microbiota by nutrient balance were clarified.

Academic Significance and Societal Importance of the Research Achievements

本研究では、タンパク質の量および質の違いのエビデンスを構築することを目標に、栄養疫学研究および基礎医学研究の融合研究により栄養バランスの代謝疾患への影響を明らかにした。動物実験だけではなくヒトでも結果をメタアナリシスで示し、さらにそのメカニズムを明らかにできれば説得性を持ってリアルワールドにダイレクトに還元可能である。近年の低炭水化物ダイエットの広がりを考慮すれば科学的エビデンス構築は喫緊の課題であり、本研究結果は日常生活での食選択を行う際の強力なエビデンスのひとつとなりうる。

Research Products

• [Presentation] Dietary Protein Quality and Quantity Changed Energy Metabolism via Liver and Intestine Interactions Signals (2018)
  • Author(s): Yoko Yokoyama, Kazuki Tanaka, Shinji Fukuda, Narumi Hanazato, Kaori Shinohara, Honoka Ijyuin, Karen Yoshimoto, Akira Honda, Kazuo Tsubota, Mitsuhiro Watanabe
  • Organizer: XXV International Bile Acid Meeting: Bile Acids in Health and Disease 2018
  • Int'l Joint Research