High efficient myogenic differentiation of human pluripotent stem cells on New Generation Laminins towards regenerative medicine
Project/Area Number |
17K13014
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
|
Research Institution | Kyoto University |
Principal Investigator |
Zhao Mingming 京都大学, iPS細胞研究所, 特定研究員 (50754206)
|
Research Collaborator |
SAKURAI Hidetoshi
SEKIGUCHI Kiyotoshi
|
Project Period (FY) |
2017-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 次世代ラミニン / iPS細胞 / 骨格筋幹細胞 / 再生医学 |
Outline of Final Research Achievements |
In this study, we aimed to use a xeno-free matrix establising a highly efficient differentiation system for inducing myocytes and muscle stem cells from hiPSCs. Instead of animal derived Matrigel, the NGL (New Generation Laminin) increases muscle progenitor cells, subsequently, increase the amounts of myocytes and muscle stem cells from hiPSCs in the stepwise differentiation protocol. Our results demonstrate that the unique crystal structure of NGL supports the highly efficient myogenic differentiation through FGF receptor signaling pathway regulated by heparan sulfates (HS). These novel effects of NGL can’t be replaced by treating with high dose of FGF2 or coating the mixture of laminin421 E8 and perlecan. Using this NGL, we established a highly efficient differentiation system for myocytes and muscle stem cells induction from hiPSCs for disease modeling and clinical application.
|
Academic Significance and Societal Importance of the Research Achievements |
Instead of animal derived matrix, using Xeno-free New Generation Laminin, we established a highly efficient differentiation system for myocytes and muscle stem cells induction from hiPSCs. This research will be very useful for disease modeling and cell therapy based on hiPSCs.
|
Report
(3 results)
Research Products
(8 results)