| Project/Area Number |
17K13026
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Yamagishi Ayana 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (00778293)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 中間径フィラメント / ネスチン / 癌転移 / ゲノム編集 / 乳癌細胞 / 癌 / 転移 |
|---|
| Outline of Final Research Achievements |
Intermediate filament nestin has a function to soften cell body, and its gene disruption reduces the invasiveness of cancer cells. In this study, we demonstrated that gene disruption of human nestin increases cellular stiffness. In addition, we established a method to disrupt the nestin gene by using vesicles containing Cas9 protein and sgRNA sequence that can knock out nestin gene highly efficiently toward suppression of cancer metastasis.
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| Academic Significance and Societal Importance of the Research Achievements |
小胞を用いて血管に侵入した癌細胞を標的としたゲノム編集技術によるネスチン遺伝子破壊が可能となれば、遠隔転移後に転移巣を形成する癌細胞の転移を抑制し、血中のリンパ球を含む免疫システムにより転移前の癌細胞を死滅させることができる。この手法が確立できれば、これまで副作用の影響が大きい化学療法でしか対処できなかった、血中循環腫瘍細胞を治療できる画期的な癌転移抑制方法に繋がることが期待できる。
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