The mechanisms of exercise-induced glucose utilization by hexokinase and mitochodria in skeletal muscle

Research Project

Project/Area Number	17K13196
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Sports science
Research Institution	Niigata University of Health and Welfare
Principal Investigator	Masuda Hiroyuki 新潟医療福祉大学, 健康科学部, 助教 (10738561)
Project Period (FY)	2017-04-01 – 2021-03-31
Project Status	Completed (Fiscal Year 2020)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	運動 / 骨格筋 / ミトコンドリア / ヘキソキナーゼ / Akt / 糖代謝 / ヘキソキナーゼ活性
Outline of Final Research Achievements	Accumulating evidence reveals that exercise stimulates glucose transport in skeletal muscle. Hexokinase phosphorylates glucose to regulate glucose metabolism. Akt is activated by exercise. In heart muscle, Akt-mediated phosphorylation of hexokinase increases mitochondrial association. This association, which include translocates to mitochondria and increases mitochondrial hexokinase binding, contributes to generate ATP. We hypothesized that Akt-mediated phosphorylation of hexokinase increases during exercise. Contrary to our hypothesis, exercise did not change mitochondrial hexokinase binding.
Academic Significance and Societal Importance of the Research Achievements	本研究は、これまでGLUT4による血糖取り込み過程に偏重していた骨格筋糖代謝研究において、ミトコンドリアでの血糖利用を調節するという新たな視点から、この研究分野を再構築することを目指した。本研究の成果は、身体活動による糖代謝機能の包括的な解明への一助となることが期待される。