Elucidation of mechanism of fermented milk suppresses exercise-induced muscle damage in skeletal muscle

• Project/Area Number: 17K13199
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Kyoto Kacho University
• Principal Investigator: Iwasa Masayo 京都華頂大学, 現代家政学部食物栄養学科, 助教 (10780041)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 運動 / 骨格筋 / 発酵乳 / 乳カゼイン加水分解物 / 糖代謝 / 筋損傷 / 酸化ストレス

Outline of Final Research Achievements

Unaccustomed and strenuous exercise causes muscle damage that clinically presents as muscular pain and involves protein degradation and ultrastructural changes, a condition known as delayed-onset muscle damage. We found that the supplementation of Lactobacillus helveticus fermented milk and milk casein hydrolysate (MCH) improve glucose metabolism and alleviate the effects of muscle soreness after exercise in human. MCH enhanced glucose uptake with activated insulin-independent signaling in skeletal muscle cells, which might be mediated by MCH-derived peptide.

Academic Significance and Societal Importance of the Research Achievements

乳カゼイン加水分解物が、AMPKを律速とするインスリン非依存性シグナル伝達の活性化を誘導し糖取り込みを亢進させることを明らかにし、乳カゼイン加水分解物由来の特異的なペプチドが関与している可能性を示した。AMPKの活性化は糖取り込みだけでなく脂肪酸の分解を高め、運動時の持久力や耐糖能に影響するため、本研究で検討したAMPKの活性を高める食品成分は、競技パフォーマンス向上や疾病予防において有用であると考えられる。

Research Products

• [Journal Article] 発酵乳由来ペプチドの骨格筋糖代謝改善作用 (2019)
  • Author(s): 岩佐真代、青井渉
  • Journal Title: 食品加工技術 Volume: 39 Pages: 1-6
  • NAID: 40022167866
  • Peer Reviewed
• [Journal Article] 中高齢者における運動の糖代謝改善作用を促す発酵乳バイオジェニックスの検討 (2017)
  • Author(s): 青井渉、岩佐真代
  • Journal Title: デサントスポーツ科学 Volume: 38 Pages: 164-171
  • NAID: 40021225696
  • Peer Reviewed
• [Presentation] The effects of milk casein hydrolysate and its derived peptides on glucose metabolism in C2C12 skeletal muscle cells (2019)
  • Author(s): Iwasa M, Miyazaki H, Aoi W
  • Organizer: 13th Asian Congress of Nutrition
  • Int'l Joint Research
• [Presentation] 乳カゼイン加水分解物由来ペプチドが骨格筋細胞における糖代謝に及ぼす影響 (2018)
  • Author(s): 岩佐真代、竹添詩奈、壽谷尭俊、宮崎秀俊、青井渉
  • Organizer: 第73回日本体力医学会大会
• [Presentation] 乳カゼイン加水分解物が中高齢者における運動後の筋損傷および糖代謝に及ぼす影響 (2017)
  • Author(s): 岩佐真代、宮﨑秀俊、青井渉
  • Organizer: 第71回日本栄養・食糧学会大会
• [Presentation] 乳カゼイン加水分解物が骨格筋細胞における糖代謝に及ぼす影響 (2017)
  • Author(s): 岩佐真代、北浦奈央子、青井渉
  • Organizer: 第72回日本体力医学会大会
• [Book] Dairy in human health and disease across the lifespan. Chapter 13, Benefits of Lactobacillus helveticus fermented milk in sports and health. (2017)
  • Author(s): Iwasa M, Aoi W
  • Total Pages: 473
  • Publisher: Academic Press