Research Project
Grant-in-Aid for Young Scientists (B)
For the purpose of searching causal SNPs which regulate blood HDL level, the TFEL scan method was used to analyze SNPs in the promoter region of apoAI. First, a reference sequence of the promoter region was inserted into one of two different luciferase reporter vectors and a sequence having only one SNP in the same region was inserted into the other vector. These vectors were co-transfected into human cell lines to evaluate the ratio of luciferase activity. Next, the TFEL scan method was used to identify transcription factors that bind to SNPs selected by screening.
同定された転写因子をヒト細胞株で過剰発現したところ、内因性のapoAI遺伝子の発現が増加した。apoAIのプロモーター領域にあり、転写活性に影響を与えるSNPと、この部位に結合してapoAI遺伝子の発現を制御する転写因子を同定した。
All 2019 2018 2017 Other
All Journal Article (8 results) (of which Int'l Joint Research: 6 results, Peer Reviewed: 8 results, Open Access: 8 results, Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)
FEBS Letters
Volume: 593 Issue: 4 Pages: 423-432
10.1002/1873-3468.13328
Scientific Reports
Volume: 9 Issue: 1 Pages: 5169-5169
10.1038/s41598-019-41631-1
120007133465
Endocrinology.
Volume: 159(6) Issue: 6 Pages: 2308-2323
10.1210/en.2017-03179
FEBS Lett.
Volume: 592(3) Issue: 3 Pages: 422-433
10.1002/1873-3468.12975
FEBS Lett
Volume: 591 Issue: 7 Pages: 965-978
10.1002/1873-3468.12620
Diabetes
Volume: 66 Issue: 7 Pages: 1833-1846
10.2337/db16-1277
Biochem Biophys Res Commun.
Volume: 493 Issue: 1 Pages: 40-45
10.1016/j.bbrc.2017.09.081
Surg Case Rep
Volume: 3 Issue: 1 Pages: 131-131
10.1186/s40792-017-0408-x
120007134592
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