| Project/Area Number |
17K13221
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Kyoto University |
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Principal Investigator |
Takata Takumi 京都大学, 複合原子力科学研究所, 准教授 (80379007)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | クリスタリン / 白内障 / アスパラギン酸 / 翻訳後修飾 / 異性化 / 加齢 / 質量分析 / MRM法 / Asp異性化 / 結合型D-アミノ酸 / D-アミノ酸 / 脱アミド化 / 加齢性疾患 / タンパク質異常凝集 / 老化 / 水晶体 / プロテオーム |
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| Outline of Final Research Achievements |
The purpose of this study is to define the spontaneous chemical alterations (Asp isomerization) of aspartic acid residues in proteins as a new aging index for proteins containing them. Asp residue in protein is isomerized during life. Along with this, isomerized Asp causes structural changes in proteins including itself, and may cause the onset of age-related diseases (cataract, Alzheimer's disease, etc.). Therefore, quantitative analysis of isomerized Asp in proteins may lead to early detection of diseases. In this study, we succeeded in developing a method for finding a very small amount of isomerized Asp present in the protein, and also succeeded in demonstrating the importance of the site where isomerization was observed.
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| Academic Significance and Societal Importance of the Research Achievements |
眼の水晶体内部における加齢分子マーカーを確立した。
水晶体内部における『タンパク質分子年齢』の提唱により、年齢や、測定した眼機能評価による組織年齢以外に、組織（水晶体）の状態や機能を蛋白質分子レベルで評価することができる。これまでにない眼機能の多次元評価が可能となり、また該当部位を疾患予想部位と定め、新たな予防法や薬剤の標的部位とすることができる。本研究で得られた成果は、80歳以上ほぼ全ての人類が罹患する、加齢性白内障の早期症状発見や発症抑制につながる。
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