• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Biomarker research for NASH diagnosis based on the disease-related glycoantigen expression in liver-resident macrophages

Research Project

Project/Area Number 17K13223
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied health science
Research InstitutionSaga University

Principal Investigator

Okada Takahiro  佐賀大学, 医学部, 助教 (30584809)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生活習慣病 / マクロファージ / 糖鎖 / バイオマーカー / 肝炎 / 疾患マーカー
Outline of Final Research Achievements

In the present study, the correlation between non-alcoholic steatohepatitis (NASH) progression and glycoantigen expression in liver-resident macrophages (Kupffer cells) was investigated. Using a mouse model, disease-related alteration of protein glycosylation was demonstrated by lectin microarray-based glycoprofiling. In association with the pathogenesis of NASH, the content of membrane protein-bound oligosaccharides harboring galactose/N-acetylgalactosamine residues increased in Kupffer cells. The results also suggested that disease progression altered the degree of oligosaccharide branching. Furthermore, the results of differential gene expression analysis suggested that the up-regulation of B3gnt5 and the down-regulation of St6gal1 were involved in such alteration.

Academic Significance and Societal Importance of the Research Achievements

NASHは早期診断の方法が確立されておらず、自覚症状を伴わないために肝がんや肝硬変への進展リスクが高い生活習慣病である。本研究で得られた結果をもとに、初期病態を反映するような糖鎖抗原の分布について詳細な検討を行っていくことで、クッパー細胞の性状変化に基づく診断バイオマーカーの開発が可能になると考えられる。これを実現化できれば、例えば血液生化学検査のように身体的・精神的負担の少ない病態鑑別が可能となり、子供から老人に至るまで幅広い年代に対して生活習慣病の早期予防を実施できるようになると期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (3 results)

All 2019 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Molecular cloning and functional expression of Lewis type α1,3/α1,4-fucosyltransferase cDNAs from Mangifera indica L.2017

    • Author(s)
      Okada T, Ihara H, Ito R, Ikeda Y
    • Journal Title

      Phytochemistry

      Volume: 144 Pages: 98-105

    • DOI

      10.1016/j.phytochem.2017.08.021

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] NASHの進展に連動した肝常在性マクロファージの糖鎖発現の変化2019

    • Author(s)
      岡田貴裕, 井原秀之, 伊東利津, 池田義孝
    • Organizer
      第92回 日本生化学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 非アルコール性脂肪性肝炎の進展に連動した肝常在性マクロファージにおけるタンパク質糖鎖修飾の変化2019

    • Author(s)
      岡田貴裕
    • Organizer
      第43回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi