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Development of a screening system for cell penetrating peptides mediated by split intein

Research Project

Project/Area Number 17K13269
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Miyafusa Takamitsu  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (70760271)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsタンパク質 / タンパク質工学 / バイオ医薬品 / バイオテクノロジー / 分子設計 / 細胞膜透過ペプチド / split intein / 蛋白質工学 / 細胞工学 / インテイン
Outline of Final Research Achievements

Protein-protein interactions in cells are the basis of life phenomena, and promising drug targets. However, it remains extremely difficult to develop the molecules that precisely control protein-protein interactions in cells. In this study, we aimed to create artificial molecules that function in cells. We worked on the development of a simple selection method for peptides that enable molecular delivery into cells. We also worked on the development of functional molecules suitable for delivery.

Academic Significance and Societal Importance of the Research Achievements

近年、開発と利用が広がっている抗体医薬品などは細胞表面の標的分子に結合して機能する分子群であり、治療の対象は本質的に制限される。一方で、細胞内で生じるタンパク質間相互作用を阻害する創薬は、より多くの分子を標的にできることから大きな期待を集めている。しかし、細胞内のタンパク質間相互作用を精緻に制御するような分子の開発は極めて困難であり、未だ基盤技術の整備が進んでいない。本研究の成果は、この困難を突破し次世代の医薬品開発の端緒となるものである。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Generation of ubiquitin-based binder with an inserted active peptide2018

    • Author(s)
      Miyafusa Takamitsu、Hirota Kiyonori、Honda Shinya
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 503 Issue: 4 Pages: 3162-3166

    • DOI

      10.1016/j.bbrc.2018.08.110

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural insights into the backbone-circularized granulocyte colony-stimulating factor containing a short connector2018

    • Author(s)
      Miyafusa Takamitsu、Shibuya Risa、Honda Shinya
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 500 Issue: 2 Pages: 224-228

    • DOI

      10.1016/j.bbrc.2018.04.045

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Backbone Circularization Coupled with Optimization of Connecting Segment in Effectively Improving the Stability of Granulocyte-Colony Stimulating Factor2017

    • Author(s)
      Miyafusa T, Shibuya R, Nishima W, Ohara R, Yoshida C and Honda S
    • Journal Title

      ACS Chem. Biol.

      Volume: 12 Issue: 10 Pages: 2690-2696

    • DOI

      10.1021/acschembio.7b00776

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 主鎖環状化サイトカインの設計と物性評価2018

    • Author(s)
      宮房 孝光、渋谷 理紗、二島 渉、大原 璃恵、吉田 昼也、本田 真也
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Backbone Circularization Coupled with Optimization of Connecting Segment in Effectively Improving the Stability of G-CSF2018

    • Author(s)
      Miyafusa T, Shibuya R, Nishima W, Ohara R, Yoshida C, Honda S.
    • Organizer
      PEGS Europe 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 主鎖環状化を利用したG-CSFの安定化改変2017

    • Author(s)
      宮房 孝光、渋谷 理紗、二島 渉、大原 璃恵、吉田 昼也、本田 真也
    • Organizer
      生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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