Neural mechanism underlying stable neural responses during a learned movement

• Project/Area Number: 17K13277
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Brain biometrics
• Research Institution: The University of Tokyo
• Principal Investigator: Ebina Teppei 東京大学, 大学院医学系研究科(医学部), 助教 (30611206)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: in vivo ホールセル記録 / 2光子イメージング / マウス運動野 / in vivoホールセル記録 / in vivo 2光子イメージング / 神経科学

Outline of Final Research Achievements

Motor learning reduces the trial-to-trial variability in neural responses during the learned behavior, but little is known how neurons generate the stable responses during the movement. In this research project, we developed a protocol to train mouse to use forelimbs to pull a lever, and also developed methods to measure the response and synaptic inputs from the same neuron during the task performance by combining cell-attached recording/two-photon imaging and whole-cell recording. Using these techniques, we will investigate the relationship between neural activity and excitatory/inhibitory synaptic inputs during a behavior.

Academic Significance and Societal Importance of the Research Achievements

本研究では、運動中のマウス大脳皮質で同一のニューロンからその活動とシナプス入力を記録する方法を開発した。
運動実行時に単一細胞へのシナプス入力を記録する方法はこれまで、その技術的困難さからほとんど研究が行われてこなかった。そのため本研究で開発した技術によって、新規の神経活動安定化のメカニズムを明らかにできると期待される。また、運動実行にかかわる神経活動安定化のメカニズム解明を通して、例えば、脳損傷後のリハビリテーションなどの効率化へとつながる事が期待される。

Research Products

• [Journal Article] Common marmoset as a model primate for study of the motor control system (2020)
  • Author(s): Matsuzaki Masanori、Ebina Teppei
  • Journal Title: Current Opinion in Neurobiology Volume: 64 Pages: 103-110
  • DOI: 10.1016/j.conb.2020.02.013
  • Peer Reviewed / Open Access
• [Journal Article] Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset (2019)
  • Author(s): Ebina Teppei、Obara Keitaro、Watakabe Akiya、Masamizu Yoshito、Terada Shin-Ichiro、Matoba Ryota、Takaji Masafumi、Hatanaka Nobuhiko、Nambu Atsushi、Mizukami Hiroaki、Yamamori Tetsuo、Matsuzaki Masanori
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Issue: 45 Pages: 22844-22850
  • DOI: 10.1073/pnas.1903445116
  • Peer Reviewed / Open Access
• [Journal Article] Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks (2018)
  • Author(s): Ebina, T., Masamizu, Y., Tanaka, YR., Watakabe, A., Hirakawa, R., Hirayama, Y., Hira, R., Terada, S., Koketsu, D., Hikosaka, K., Mizukami, H., Nambu, A., Sasaki, E., Yamamori, T. and Matsuzaki
  • Journal Title: Nature Communications Volume: 印刷中
  • Peer Reviewed / Open Access
• [Presentation] Fluorescent glass pipettes for optically targeted electrophysiological recordings. (2019)
  • Author(s): 1.Okamoto, K., Fuji, N., Ebina, T., Konishi, K., Sato, Y., Kashima, T., Nakano, R., Hioki, H., Takeuchi, H., Yumoto, J., Matsuzaki, M. and Ikegaya, Y.
  • Organizer: Neuroscience 2019
  • Int'l Joint Research
• [Presentation] Calcium imaging of the auditory mismatch negativity (aMMN) responses in common marmosets. (2019)
  • Author(s): 2.Obara, K., Ebina, T., Masamizu, Y., Terada, S., Uka, T., Komatsu, M., Ichinohe, N., Watakabe, A., Mizukami, H., Yamamori, T., Kasai, K. and Matsuzaki, M.
  • Organizer: The 42th Annual Meeting of the Japan Neuroscience Society
• [Presentation] Upper-limb movements induced by optogenetic stimulation of the motor cortex in the common marmoset. (2019)
  • Author(s): 3.Ebina, T., Obara, K., Masamizu, Y., Terada, S., Watakabe, A., Matoba R., Hatanaka, N., Nambu, A., Mizukami, H., Yamamori, T. and Matsuzaki, M.
  • Organizer: The 42th Annual Meeting of the Japan Neuroscience Society
• [Presentation] Two-photon imaging of neuronal activity in motor cortex of non-human primate during reaching tasks (2019)
  • Author(s): Ebina, T., Masamizu, Y., Obara, K., and Matsuzaki, M.
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies congress
  • Int'l Joint Research
• [Presentation] テルビウムドープガラスの蛍光を手がかりにした標的パッチクランプ法 (2019)
  • Author(s): 岡本和樹, 藤井直樹, 蝦名鉄平, 小西邦昭, 佐藤由宇, 中野利沙子, 日置寛之, 山口瞬, 竹内春樹, 湯本潤司, 松崎政紀, 池谷裕二
  • Organizer: 日本薬学会第139年会
• [Presentation] Two-photon imaging of neuronal activity in the motor cortex of marmosets during reaching tasks. (2019)
  • Author(s): Ebina, T., Masamizu, Y., Tanaka, YR., Watakabe, A., Hirakawa, R., Hirayama, Y., Hira, R., Terada, S., Koketsu, D., Hikosaka, K., Mizukami, H., Nambu, A., Sasaki, E., Yamamori, T. and Matsuzaki, M.
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] Functional imaging of the auditory cortex in common marmosets: For elucidating the mechanisms of the auditory mismatch negativity (2019)
  • Author(s): Obara, K., Ebina, T., Masamizu, Y., Uka, T., Komatsu, M., Ichinohe, N., Watakabe, A., Mizukami, H., Yamamori, T., Kasai, K., and Matsuzaki, M.
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] Two-photon imaging of neuronal activity in motor cortex of common marmosets during reaching tasks (2018)
  • Author(s): Ebina, T.
  • Organizer: 国際ワークショップ「遺伝子導入技術の利用による霊長類脳機能操作とイメージング」
  • Invited
• [Presentation] Two-photon calcium imaging in the motor cortex of common marmosets during upper-limb movement tasks. (2018)
  • Author(s): Masamizu, Y., Ebina, T., and Matsuzaki, M.
  • Organizer: The 41st Annual Meeting of the Japan Neuroscience Society
• [Presentation] Two-photon imaging of neuronal activity in motor cortex of common marmosets during reaching tasks (2018)
  • Author(s): Ebina, T.
  • Organizer: Joint symposium of 10th Optogenetics Research Conference and Second International Symposium on Brain Information Dynamics
• [Presentation] Two-photon imaging of neuronal activity in motor cortex of common marmosets during upper-limb movement behavior tasks (2018)
  • Author(s): Ebina, T., Masamizu, Y., and Matsuzaki, M.
  • Organizer: Neuroscience 2018
  • Int'l Joint Research
• [Presentation] Two-photon imaging in primary motor cortex during a forelimb movement task in the head-restrained common marmoset (2017)
  • Author(s): 1.Ebina, T., Masamizu, Y., Hirakawa, R., Watakabe, A., Ohkura, M., Kobayashi, K., Koketsu, D., Nambu, A., Hikosaka, K., Sasaki, E., Nakai, J., Yamamori, T. and Matsuzaki, M.
  • Organizer: The 40th Annual Meeting of the Japan Neuroscience Society