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Femtosecond laser surface modification of a capillary flow immunoassay microchip for spatially-selected antibody immobilization and its mechanism

Research Project

Project/Area Number 17K14140
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Quantum beam science
Research InstitutionAkita Prefectural University (2019)
Osaka University (2017-2018)

Principal Investigator

Kenji Goya  秋田県立大学, システム科学技術学部, 助教 (40757332)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsフェムト秒レーザー加工 / 表面改質 / 抗体固定化 / マイクロ流体チップ / 超親水性 / フェムト秒レーザー / レーザー加工 / PMMA / 親水化 / フェムト秒レーザー微細加工 / 材料親水化 / フェムト秒レーザ加工 / 表面修飾
Outline of Final Research Achievements

We have developed a localized antibody immobilization technique using by femtosecond laser micro-grooving. To be clear the immobilization mechanism, the mechanical and chemical properties and the amount of immobilized antibody varying with laser irradiation parameters have experimentally been investigated.

Academic Significance and Societal Importance of the Research Achievements

生化学分析法の技術向上に資する技術であると期待する。特に抗体検査においては、本技術は樹脂材料表面にレーザー照射をするだけで高精度な抗体固定化を達成するため、クリーンで低コストでありながら、マイクロ流体チップのような狭い空間に検出点を集積可能であるため、特定の検査項目については大きな利点が得られる。
また、レーザー照射が材料表面を改質した際の機序を明らかにすることでレーザー加工技術の高度化・メカニズム解明に貢献する。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Paper-like Surface Microstructure Fabricated on a Polymer Surface by Femtosecond Laser Machining2018

    • Author(s)
      Kenji Goya, Yusuke Fuchiwaki
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 1 Pages: 33-38

    • DOI

      10.2116/analsci.34.33

    • NAID

      130006306748

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Femtosecond laser surface modification of an immunoassay microchip for spatially-localized antibody immobilization2017

    • Author(s)
      Kenji Goya, Yusuke Fuchiwaki, Masato Tanaka, Toshihiko Ooie
    • Journal Title

      Proceeding of Laser Precision Microfabrication

      Volume: A180 Pages: 1-4

    • Related Report
      2017 Research-status Report
  • [Presentation] フェムト秒レーザ加工を利用したマイクロ流体チップの局所親水化と抗体分子の固定化2019

    • Author(s)
      合谷賢治、渕脇雄介、田中正人、大家俊彦、片岡正俊
    • Organizer
      レーザー学会学術講演会 第40回年次大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] フェムト秒レーザアブレーションを利用した PMMA基板の親水化と抗体タンパク質の局所固定化2018

    • Author(s)
      合谷賢治、渕脇雄介、田中正人、大家利彦
    • Organizer
      応用物理学会春季学術講演会2018
    • Related Report
      2017 Research-status Report
  • [Presentation] Femtosecond laser surface modification of an immunoassay microchip for spatially-localized antibody immobilization2017

    • Author(s)
      Kenji Goya, Yusuke Fuchiwaki, Masato Tanaka, Toshihiko Ooie
    • Organizer
      The 18th International Symposium on Laser Precision Microfabrication (LPM)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 秋田県立大学 教員紹介

    • URL

      http://www.akita-pu.ac.jp/stic/souran/scholar/detail.php?id=350

    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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