Spatiotemporal formation of amyloid assembly by optical trapping and fluorescence analysis of its dynamics

• Project/Area Number: 17K14427
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: Yuyama Ken-ichi 北海道大学, 電子科学研究所, 助教 (20786355)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 光ピンセット / 光圧 / アミロイド / タンパク質 / 顕微分光 / 顕微イメージング / レーザー / ナノバイオ / 顕微計測

Outline of Final Research Achievements

We successfully demonstrated spatially- and temporally- controlled formation of an amyloid assembly by optical trapping of partially unfolded proteins in solution. The fluorescence analysis during the trapping process revealed that the formation of an amyloid assembly proceeds in two steps; aggregation of proteins and their amyloid fibrillation. It was found that the assembly formed in the first step consists of the entangled single fibrillar structure. Thus, it was implied that intermediate proteins prior to amyloid nucleation can form the fibrillar structure like amyloid during their assembling process.

Academic Significance and Societal Importance of the Research Achievements

アミロイドは、様々な疾患と関係している不溶性のタンパク質凝集体である。確率的に起こるアミロイド核形成を溶液中で時空間制御することは難しく、溶液全体のアンサンブル測定を通してその形成過程が調べられてきた。本研究で示した新しいアミロイド集合体形成手法は、一集合体レベルでの解析を可能にし、アミロイド線維形成メカニズムの解明に大きく貢献できると期待される。

Research Products

• [Int'l Joint Research] 国立交通大学（台湾）(その他の国・地域)
• [Int'l Joint Research] 国立交通大学(台湾)
• [Journal Article] Bubble generation and molecular crystallization at solution surface by intense continuous-wave laser irradiation (2018)
  • Author(s): Jim Jui-Kai Chen, Ken-ichi Yuyama, Teruki Sugiyama, Hiroshi Masuhara
  • Journal Title: Applied Physics Express Volume: 11 Issue: 8 Pages: 085502-085502
  • DOI: 10.7567/apex.11.085502
  • NAID: 120006709278
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pseudopolymorph Control of L-Phenylalanine Achieved by Laser Trapping (2018)
  • Author(s): Chi-Shiun Wu, Pei-Yun Hsieh, Ken-ichi Yuyama, Hiroshi Masuhara, Teruki Sugiyama
  • Journal Title: Crystal Growth & Design Volume: 18 Issue: 9 Pages: 5417-5417
  • DOI: 10.1021/acs.cgd.8b00796
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Crystallization of Methylammonium Lead Halide Perovskites by Optical Trapping (2018)
  • Author(s): Yuyama Ken-ichi、Islam Md Jahidul、Takahashi Kiyonori、Nakamura Takayoshi、Biju Vasudevanpillai
  • Journal Title: Angewandte Chemie International Edition Volume: 57 Issue: 41 Pages: 13424-13428
  • DOI: 10.1002/anie.201806079
  • NAID: 120006729575
  • Peer Reviewed
• [Journal Article] Crystal Growth and Dissolution Dynamics of L-Phenylalanine Controlled by Solution Surface Laser Trapping (2018)
  • Author(s): Ken-ichi Yuyama, Ding-Shiang Chiu, Yen-En Liu, Teruki Sugiyama, Hiroshi Masuhara
  • Journal Title: Crystal Growth & Design Volume: 18 Issue: 11 Pages: 7079-7079
  • DOI: 10.1021/acs.cgd.8b01233
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Rapid localized crystallization of lysozyme by laser trapping (2018)
  • Author(s): Ken-ichi Yuyama, Kai-Di Chang, Jing-Ru Tu, Hiroshi Masuhara, Teruki Sugiyama
  • Journal Title: Physical Chemistry Chemical Physics Volume: 20 Issue: 9 Pages: 6034-6039
  • DOI: 10.1039/c7cp06990a
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Femtosecond-Laser-Enhanced Amyloid Fibril Formation of Insulin (2017)
  • Author(s): Tsung-Han Liu, Ken-ichi Yuyama, Takato Hiramatsu, Naoki Yamamoto, Eri Chatani, Hiroshi Miyasaka, Teruki Sugiyama, Hiroshi Masuhara
  • Journal Title: Langmuir Volume: 33 Issue: 33 Pages: 8311-8318
  • DOI: 10.1021/acs.langmuir.7b01822
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 光トラッピングによる混合ハロゲン化物ペロブスカイトの結晶化 (2019)
  • Author(s): 柚山健一、イスラムジャヒダル、ビジュヴァスデヴァンピライ
  • Organizer: 日本化学会第99春季年会2019
• [Presentation] 界面での光捕捉によるハロゲン化鉛ペロブスカイトの合成と改変 (2019)
  • Author(s): 柚山健一、イスラムジャヒダル、ビジュヴァスデヴァンピライ
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] Laser trapping of hen egg-white lysozyme under acidic conditions (2018)
  • Author(s): Ken-ichi Yuyama, Md Jahidul Islam, Vasudevanpillai Biju
  • Organizer: The 19th RIES-Hokudai International Symposium
  • Int'l Joint Research
• [Presentation] 光圧によるアミノ酸、タンパク質の結晶成長制御 (2018)
  • Author(s): 杉山輝樹、柚山健一
  • Organizer: 第47回結晶成長国内会議
  • Invited
• [Presentation] Crystallization of methylammonium lead halide perovskites spatiotemporally controlled by optical trapping (2018)
  • Author(s): Ken-ichi Yuyama, Md Jahidul Islam, Vasudevanpillai BIJU
  • Organizer: 18th International Symposium on Advanced Organic Photonics (ISAOP-18)
  • Int'l Joint Research / Invited
• [Presentation] 酸性条件下でのニワトリ卵白リゾチームの光捕捉挙動 (2018)
  • Author(s): 柚山健一、イスラムジャヒダル、ビジュヴァスデヴァンピライ
  • Organizer: 2018年光化学討論会
• [Presentation] 気液界面への連続発振レーザー集光照射による有機無機ハイブリッドペロブスカイト化合物の結晶化 (2018)
  • Author(s): 柚山健一、ビジュヴァスデヴァンピライ
  • Organizer: 日本化学会第98春季年会2018
• [Presentation] 集光レーザーの光圧を用いたペロブスカイト前駆体溶液表面での光捕捉 (2018)
  • Author(s): 柚山健一、ビジュヴァスデヴァンピライ
  • Organizer: 「光圧によるナノ物質操作と秩序の創生」第２回公開シンポジウム
• [Presentation] Crystallization of CH3NH3PbBr3 by Optical Trapping at Solution Surface (2017)
  • Author(s): Ken-ichi Yuyama、Vasudevan Pillai BIJU
  • Organizer: The 18th RIES-Hokudai International Sympoium
  • Int'l Joint Research
• [Presentation] 界面での半導体ナノ材料の光トラッピング (2017)
  • Author(s): 柚山健一、ビジュヴァスデヴァンピライ
  • Organizer: 2017年光化学討論会
• [Presentation] 集光レーザーの放射圧を用いたタンパク質の光捕捉とアミロイド集合体形成 (2017)
  • Author(s): 柚山健一、ビジュヴァスデヴァンピライ
  • Organizer: 第5回アライアンス若手研究交流会
• [Presentation] 集光レーザーの放射圧を用いた分子・ナノ粒子集合体の時空間形成 (2017)
  • Author(s): 柚山健一
  • Organizer: 第33回ライラックセミナー・第23回若手研究者交流会
  • Invited
• [Remarks] 北海道大学電子科学研究所分子フォトニクス研究分野ホームページ
  • URL: http://bijulab.main.jp/en/