| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Novel methods and systems to carry out genetic testing operations (i.e., DNA extraction, gene amplifier, and detection) with a compact-disk (CD)-type microfluidic device were developed. A CD-type fluorescence system allowed us to continuously measure the fluorescence intensity in the detection chamber on the CD-type microfluidic device during rotation. A small-sized genetic testing system for loop-mediated isothermal amplification (LAMP) was successfully used for real-time measurement of the change in fluorescence intensities with gene amplification. A CD-type DNA extraction system equipped with an indium tin oxide (ITO) heater enabled us to easily perform DNA extraction on a CD-type microfluidic device. A portable and automatic gene testing system with a CD-type microfluidic device would be realized by integrated these downsized systems.
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