| Project/Area Number |
17K14517
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
INABA Hiroshi 鳥取大学, 工学研究科, 助教 (00778011)
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| Research Collaborator |
MATSUURA kazunori
KAKUGO akira
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 微小管 / チューブリン / ペプチド / Tau / 分子内包 / 金属ナノ粒子 / 生体材料 / ナノマテリアル / ナノバイオ / ナノ材料 |
|---|
| Outline of Final Research Achievements |
Microtubules are major components of cytoskeleton, which are tubular architectures with 15 nm inner diameter consisting of tubulin protein dimers. In this work, we developed a Tau-derived peptide (TP) which binds to the inner surface of microtubules and encapsulated metal nanoparticles by using TP. We designed four peptides and showed that one of the peptides could bind to the inside of microtubules. By using the peptide, gold nanoparticles and CoPt nanoparticles were encapsulated inside microtubules. Notably, structures and orientation of microtubules were modulated by the encapsulation. In summary, we established a proof of concept for molecular encapsulation inside microtubules to create microtubule-based nanomaterials.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、チューブ状タンパク質集合体である微小管の内部空間に着目し、その内部空間に分子を導入するためのペプチドの開発に成功した。このペプチドを用いることで様々な金属ナノ粒子の微小管への内包を達成し、これら内包により微小管の構造や配向を制御できることを見出した。微小管からなるナノマテリアル開発に新たな展開を示すとともに、微小管を標的とした薬剤設計や、生体環境における微小管の理解につながる基盤技術を構築した。
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