The mechanisms of extinction of fear memory by dynamism analysis and manipulation of memory engram.

• Project/Area Number: 17K14930
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo
• Principal Investigator: Kim Ryang 東京大学, 大学院医学系研究科(医学部), 助教 (60793916)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 恐怖記憶消去 / 記憶痕跡 / 光遺伝学 / 恐怖記憶 / Arc / 神経科学 / カルシウムイメージング

Outline of Final Research Achievements

The aim of this study is to identify the mechanisms of fear memory extinction from the memory engram level. Using a transgenic mouse line that can label and manipulate memory engram cells, and optogenetics, we found the brain regions which control both fear and extinction, or either fear or extinction.

Academic Significance and Societal Importance of the Research Achievements

恐怖と消去の両方を担う神経細胞集団が存在するという可能性は、この細胞集団に着目し、転換を制御し、消去誘導に関わる分子実態を明らかにする研究に発展する可能性があり、このことは、消去が心的外傷後ストレス障害といった疾患の治療標的として注目されているが、将来有効な治療薬開発の基礎研究として発展する可能性もある。

Research Products

• [Journal Article] Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses (2017)
  • Author(s): K. Inokuchi, F. Imamura, H. Takeuchi, R. Kim, H. Okuno, H. Nishizumi, H. Bito, T. Kikusui, H. Sakano
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 15977-15977
  • DOI: 10.1038/ncomms15977
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Specification of a remote memory cell ensemble during cortical tagging. (2018)
  • Author(s): Ryang Kim
  • Organizer: The 17th Annual MCCS Symposium. San Diego Convention Center, San Diego, CA, USA, November 2, 2018.
  • Int'l Joint Research / Invited
• [Presentation] 皮質領域における遠隔記憶の細胞・分子機構 (2018)
  • Author(s): 金 亮
  • Organizer: 平成30年記憶研究会、生理学研究所(岡崎)、2018年9月19‐20日
  • Invited
• [Presentation] Specification of a remote memory cell ensemble during cortical tagging. (2018)
  • Author(s): Ryang Kim, Yayoi Kondo, Takashi Kawashima, Michiko Okamura, Masatoshi Inoue, Yuichiro Ishii, Kasumi Inokuchi, Kazuki Sakai, Mio Nonaka, Masayuki Sakamoto, Hiroyuki Okuno, Haruhiko Bito.
  • Organizer: Society for Neuroscience 2018, San Diego Convention Center, San Diego, CA, USA, November 3-7, 2018.
  • Int'l Joint Research
• [Presentation] Deciphering Ca2+-dependent signaling pathways underlying cognitive processes. (2018)
  • Author(s): Bito H. Kim R, Inoue M, Inokuchi K, Yokoyama T, Sakai K, Miyazawa Y, Ishii Y, Okamura M, Gammon N, Kobari S, Kondo Y, Sakamoto M, Fujii H.
  • Organizer: 95th Annual Meeting of the Physiological Society of Japan
• [Presentation] Specification of a remote memory cell ensemble during cortical tagging through activity dependent Arc signaling (2017)
  • Author(s): Ryang Kim, Kazuki Sakai, Takashi Kawashima, Yayoi Kondo, Masatosih Inoue, Mio Nonaka, Masayuki Sakamoto, Michiko Okamura, Manabu Abe, Kenji Sakimura, Itaru Imayoshi, Hiroyuki Okuno, Haruhiko Bito
  • Organizer: 第40回日本神経科学大会
• [Presentation] Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odor-induced attractive social responses (2017)
  • Author(s): Kasumi Inokuchi, Fumiaki Imamura F, Haruki Takeuchi, Ryang Kim, Hiroyuki Okuno, Hirofumi Nishizumi, Haruhiko Bito, Takefumi Kikusui, Hitoshi Sakano
  • Organizer: The 48th Annual Meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Rational engineering of XCaMPs, a multicolor GECI suite for In vivo imaging of brain circuit dynamics (2017)
  • Author(s): Inoue M, Takeuchi A, Manita S, Horigane S, Kawakami R, Yamaguchi K, Sakamoto M, Yokoyama H, Kim R, Takemoto-Kimura S, Abe M, Yokoyama T, Quirin S, Ramakrishnan C, Sakimura K, Nemoto T, Kano M, Fujii H, Deisseroth K, Kitamura K, and Bito H
  • Organizer: The 48th Annual Meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odor-induced attractive social responses (2017)
  • Author(s): Kasumi Inokuchi, Fumiaki Imamura F, Haruki Takeuchi, Ryang Kim, Hiroyuki Okuno, Hirofumi Nishizumi, Haruhiko Bito, Takefumi Kikusui, Hitoshi Sakano
  • Organizer: 第60回日本神経化学会大会
• [Presentation] Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odor-induced attractive social responses (2017)
  • Author(s): Kasumi Inokuchi, Fumiaki Imamura F, Haruki Takeuchi, Ryang Kim, Hiroyuki Okuno, Hirofumi Nishizumi, Haruhiko Bito, Takefumi Kikusui, Hitoshi Sakano
  • Organizer: 第40回日本神経科学大会