Research Project
Grant-in-Aid for Young Scientists (B)
Single cell protein analysis is a key technology for understanding of tissue and cell heterogeneity. Mass spectrometry based proteomics is possible to comprehensively identify and quantify proteins. However, it is hardly possible to perform single cell proteomics due to low sample recovery by adsorption loss during sample preparation. To reduce the contact area of a sample solution with a plastic tube, we have developed a water droplet in oil (WinO) protocol. The number of identified peptides and signal intensity in WinO were increased 1.7-fold than those in In-solution protocol. In addition, the peptide recovery was improved by optimization of protease amount, organic solvent, beads and component of surfactants in water droplet.
同一細胞群内における分子レベルの不均一性が報告されている。細胞の個性は、幹細胞から多様な細胞種に分化を促すなど、生命活動には欠かすことができない。一方、細胞の個性は腫瘍細胞に抗がん剤耐性を付与しガンの根治を難しくするなど、人類が生きる上でネガティブな一面も持ち合わせている。タンパク質は生命活動の中心的役割を担う。1細胞プロテオミクスを実現できれば、赤芽球分化様式の解明だけでなく、腫瘍細胞集団内の抗がん剤耐性細胞やガン幹細胞など希少細胞集団に効果的な薬剤の開発、ヒト造血幹細胞の増殖法の開発など幅広い分野に利用されることが期待される。
All 2018 2017
All Journal Article (3 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 3 results, Open Access: 2 results) Presentation (18 results) (of which Int'l Joint Research: 4 results, Invited: 5 results) Patent(Industrial Property Rights) (1 results)
Cancer Cell.
Volume: 33 Issue: 3 Pages: 386-400
10.1016/j.ccell.2018.01.012
Journal of Biochemistry
Volume: 162 Issue: 6 Pages: 391-394
10.1093/jb/mvx058
40021421272
臨床化学
Volume: 46 Pages: 283-290
