| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
The role of autophagy in the degradation of proteins and organelles is well-characterized. However, the autophagy-mediated RNA degradation in response to stress and the potential preference of such RNA degradation have not been examined. In this study, we analyzed selective mRNA and tRNA degradation by autophagy in yeast. Profiling of mRNAs delivered to the vacuole reveals that a subset of mRNAs is preferentially delivered to the vacuole by autophagy where it is subsequently degraded. mRNA delivery to the vacuole is associated with translation: genome-wide ribosome profiling revealed a high correspondence between ribosome association and targeting to the vacuole. Moreover, tRNA is also preferentially delivered to the vacuole. We found that aminoacylation of tRNA has important role in alteration of selectivity. We propose that autophagy-mediated mRNA and tRNA degradation is a previously-unappreciated function of autophagy that affords post-transcriptional regulation.
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