Structure determination and pressure effects of major and minor conformations of phosphorylated ubiquitin
| Project/Area Number |
17K15078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Ubiquitin / リン酸化Ubiquitin / 構造揺らぎ / 高圧力NMR / リン酸化ユビキチン / 高圧力 / NMR / 構造生物学 / 圧力 / 構造解析 / 構造揺らぎ機能相関研究 / パーキンソン病 |
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| Outline of Final Research Achievements |
Ubiquitin phosphorylated at Ser65 (pUb) is Parkin activator. Interestingly, two sets of NMR signals were observed in HSQC spectrum of pUb. The major form has a closely similar conformation to the wild type. On the other hand, the minor form has entirely different hydrogen bonding patterns at b-sheet region, in which b5-strand has retracted into the ubiquitin core region by two amino acid residues. The retracted minor conformation provides different interaction surface on the ubiquitin hydrophobic patch from that of the major one. Here, we report the analysis of conformational fluctuation of pUb using high-pressure NMR spectroscopy. Our results indicate that pressure-induced chemical shifts were markedly different at the b-sheet and a-helix regions between the major and minor ones, indicating that pressure-induced changes in hydrogen bond length and torsion angles are different at the b-sheet region.
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| Academic Significance and Societal Importance of the Research Achievements |
リン酸化ユビキチンは非リン酸化ユビキチンで機能していた一部のリガンドとの相互作用能が喪失していることが知られている。そのため、リン酸化ユビキチンと様々なリガンドとの相互作用とmajor-minor間の構造揺らぎの関係は興味深く、高圧力NMR法により解明されたminor状態転移への中間状態はその知見の一つになることが期待される。
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Report
(5 results)
Research Products
(15 results)
