| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
We attempted to identify and characterize an unknown phospholipid scramblase molecule that acts on phosphatidylserine (PS) exposure by 3'-O- (4-Benzoylbenzoyl) adenosine 5'-triphosphoric acid (BzATP). We tried a genome-wide CRISPR screen for molecules responsible for BzATP-induced PS exposure with flow cytometry for cell sorting and a next-generation sequence. As a result, we identified a transmembrane protein, essential for reactive oxygen species (Eros), as a necessary component for P2X7 expression on the cell membrane and found that it is an important molecule for PS exposure by BzATP, similar to P2X7.
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