Establishing "Architectural RNA" as a novel functional category of noncoding RNAs

• Project/Area Number: 17K15084
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Kumamoto University (2018-2019); Hokkaido University (2017)
• Principal Investigator: Chujo Takeshi 熊本大学, 大学院生命科学研究部(医), 特任助教 (50788578)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: architectural RNA / 核内RNA顆粒 / 難溶性RNA / Sam68 nuclear body / FABS / Architectural RNA / 長鎖noncoding RNA / prion-like domain

Outline of Final Research Achievements

This study, along with my successor's study, other lab members' studies, and my collaborator's studies have collectively enabled the establishment of "architectural RNA" as one functional category of long noncoding RNAs, which was the final goal of this study.

Academic Significance and Societal Importance of the Research Achievements

今世紀に発見された機能未知のnoncoding RNA (ncRNA) 群の内、核内顆粒の構造構築を司るncRNAをarchitectural RNA (arcRNA) として確立することができた。

Research Products

• [Journal Article] Regulation of growth hormone biosynthesis by Cdk5 regulatory subunit associated protein 1-like 1 (CDKAL1) in pituitary adenomas (2019)
  • Author(s): Takesue Y, Wei F-Y, Tomizawa K, Morioka M et al.,
  • Journal Title: Endocrine Journal Volume: 66 Issue: 9 Pages: 807-816
  • DOI: 10.1507/endocrj.EJ18-0536
  • NAID: 130007719407
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access
• [Journal Article] Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation (2018)
  • Author(s): Yamazaki Tomohiro、Souquere Sylvie、Chujo Takeshi、Kobelke Simon、Chong Yee Seng、Fox Archa H.、Bond Charles S.、Nakagawa Shinichi、Pierron Gerard、Hirose Tetsuro
  • Journal Title: Molecular Cell Volume: 70 Issue: 6 Pages: 1038-1053
  • DOI: 10.1016/j.molcel.2018.05.019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nuclear Bodies Built on Architectural Long Noncoding RNAs: Unifying Principles of Their Construction and Function. (2017)
  • Author(s): Takeshi Chujo and Tetsuro Hirose
  • Journal Title: Molecules and Cells Volume: 40 Pages: 889-896
  • Peer Reviewed / Open Access
• [Presentation] tRNAメチル化酵素による翻訳制御とその破綻による精神遅滞発症の分子機構 (2019)
  • Author(s): 中條 岳志、永芳 友、鈴木 健夫、Chien Wen Chen、平田 翔児、Yi Kai Chen、田中 元雅、鈴木 勉、富澤 一仁、魏 范研
  • Organizer: 日本RNA学会年会
• [Presentation] tRNAメチル化酵素の機能欠損による疾患発症機構の解明 (2019)
  • Author(s): 中條岳志
  • Organizer: 2018年度健康長寿代謝制御研究センター交流会
• [Presentation] 翻訳マシナリ解析技術の最先端とその神経疾患機序解明への応用 (2018)
  • Author(s): 中條岳志
  • Organizer: 健康長寿代謝制御センター神経感覚運動器研究部門交流セミナー
• [Presentation] architectural RNA配列の特徴抽出 (2018)
  • Author(s): 天津早貴, 岡田航, 曽超, 中條岳志, 廣瀬哲郎, 浜田道昭
  • Organizer: 生命科学系フロンティアミーティング2018
• [Presentation] Functional domains of NEAT1 architectural lncRNA induce paraspeckle assembly through phase separation (2018)
  • Author(s): Tomohiro Yamazaki, Sylvie Souquere, Hyura Yoshino, Hiro Takakuwa, Takeshi Chujo, Archa Fox, Charles Bond, Shinichi Nakagawa, Gerard Pierron, Tetsuro Hirose
  • Organizer: The Joint Symposium of the 13th International Symposium of the Institute Network for Biomedical Sciences and the 28th Hot Spring Harbor International Symposium 2018
  • Int'l Joint Research
• [Presentation] Multiple NEAT1 modular RNA domains cooperatively construct phase-separated, distinct, highly ordered paraspeckle nuclear bodies (2018)
  • Author(s): Tomohiro Yamazaki, Sylvie Souquere, Hiro Takakuwa, Hyura Yoshino, Takeshi Chujo, Archa Fox, Charles Bond, Shinichi Nakagawa, Gerard Pierron, and Tetsuro Hirose
  • Organizer: JAJ RNA 2018
  • Int'l Joint Research
• [Presentation] 核内構造体アーキテクチュラルRNAの探索手法の開発：難溶性RNA-seqとFABS法 (2018)
  • Author(s): 中條岳志、田中くみ子、山崎智弘、萬年太郎、川口哲哉、黒坂哲、内匠透、中川真一、廣瀬哲郎
  • Organizer: 第3回北海道大学・部局横断シンポジウム
• [Presentation] Unusual semi-extractability as a hallmark of nuclear body-associated architectural long noncoding RNAs (2017)
  • Author(s): Takeshi Chujo, Tomohiro Yamazaki, Tetsuya Kawaguchi, Satoshi Kurosaka, Toru Takumi, Shinichi Nakagawa, and Tetsuro Hirose
  • Organizer: RNA Society Meeting 2017
  • Int'l Joint Research
• [Presentation] 核内構造体アーキテクチュラルRNAの探索手法の開発：難溶性RNA-seqとFABS法 (2017)
  • Author(s): 中條岳志、田中くみ子、山崎智弘、萬年太郎、川口哲哉、黒坂哲、内匠透、中川真一、廣瀬哲郎
  • Organizer: 第8回北海道大学遺伝子病制御研究所研究成果発表会
• [Presentation] Unusual semi-extractability as the hallmark of nuclear body-associated architectural long ncRNAs (2017)
  • Author(s): Takeshi Chujo, Tomohiro Yamazaki, Tetsuya Kawaguchi, Satoshi Kurosaka, Toru Takumi, Shinichi Nakagawa, Tetsuro Hirose
  • Organizer: 日本RNA学会年会
• [Presentation] 核内構造体形成のアーキテクチュラル長鎖ncRNAの共通特性としての難抽出性 (2017)
  • Author(s): 中條岳志、山崎智弘、川口哲哉、黒坂哲、内匠透、中川真一、廣瀬哲郎
  • Organizer: 生化学会北海道支部会
• [Presentation] nusual semi-extractability as a hallmark of nuclear body-associated architectural long ncRNAs (2017)
  • Author(s): Takeshi Chujo and Tetsuro Hirose
  • Organizer: 2nd International Symposium for Noncoding RNA Neo-taxonomy
  • Int'l Joint Research
• [Remarks] 「抽出しにくいRNA」から核内顆粒形成に関わる癌関連ノンコーディングRNAを発見
  • URL: https://www.hokudai.ac.jp/news/170413_pr.pdf