Elucidation of RNA recognition mechanism of Xrn1
| Project/Area Number |
17K15092
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Okayama University |
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Principal Investigator |
Watanabe Kazunori 岡山大学, ヘルスシステム統合科学研究科, 助教 (70602027)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | Xrn1 / Xrn2 / 熱ストレス / miRNA / tRNA分解 / miRNA分解 / ストレス応答 |
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| Outline of Final Research Achievements |
In this study, we attempted to elucidate the activation mechanism and RNA recognition mechanism of Xrn1 and Xrn2 (Xrn1/2) by heat stress. At first, to elucidate the RNA recognition mechanism of Xrn1/2, we examined the construction and purification condition of Xrn1/2 expression system using various affinity tags. As a result, the yield and purity of Xrn1/2 could not be sufficiently obtained. However, Xrn2 activity could be confirmed in in vitro analysis.
In order to clarify the activation mechanism of Xrn1/2, we attempted to determine the proteins that bind to and dissociate from Xrn1/2 in heat-dependent manner. We succeeded in identifying multiple proteins. We are currently investigating whether these proteins are involved in the heat-induced activation of Xrn1/2.
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| Academic Significance and Societal Importance of the Research Achievements |
熱ストレス依存的なXrn1/2結合・解離タンパク質を同定することで、Xrn1/2の活性化機構を明らかにできると考えている。今後、Xrn1/2活性化機構を明らかにしていくことで、ストレス応答研究の基礎的な知見として貢献できると考えている。また、がん治療法の１つである温熱療法の基礎的な知見としても貢献できるだけでなく、より効果的な温熱療法開発研究へと普及していくと考えている。
またin vitro解析よりXrn2は開始tRNAを分解できたことから、細胞内で観察されていたXrn2の活性は正しかったことが裏付けされた。そのため、Xrn2の新たな基質としての知見として貢献できたと考えている。
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Endosomal escape of peptide-photosensitizer conjugates is affected by amino acid sequences near the photosensitizer.2020
Author(s)
Miyoshi, Y., Kadono, M., Okazaki, S., Nishimura, A., Kitamatsu, M., Watanabe, K., and Ohtsuki, T.
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Journal Title
Bioconjugate Chemistry
Volume: 31
Issue: 3
Pages: 916-922
DOI
Related Report
Peer Reviewed
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[Presentation] Photochemical and sonochemical internalization of CPP-cargo-sensitizer conjugates2018
Author(s)
T. Ohtsuki, S. Miki, Y. Inaba, S. Kobayashi, T. Haraguchi, E. Nakata, A. Harada, K. Hirakawa, S. Okazaki, M. Kitamatsu, K. Watanabe
Organizer
Pacific Rim Nano Medicine Symposium
Related Report
Int'l Joint Research
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