Analysis of robustness for organ size control

• Project/Area Number: 17K15127
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Akiyama Ryutaro 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (00790403)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: クッペル胞 / 器官形成 / 器官サイズ / FGF / ロバスト性

Outline of Final Research Achievements

In this study, we aimed to elucidate a novel type of organ robustness where the fluctuations of organ size are compensated at the functional level.Using the zebrafish Kupffer's vesicle as a model system, we performed simultaneous evaluation of organ size and function, and combined them with live imaging.We propose that the Kupffer’s vesicle maintains its function by fine-tuning the cilium property according to organ size.

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた成果は、生命が個体や器官の大きさをどのように制御・維持しているのかについて、特に機能との関連からその仕組みを考える上で、重要な知見となる可能性がある。また、本研究による器官サイズ制御機構の理解は、再生医療に必須な「器官の大きさを自由に操る技術」などの応用面への展開が期待される。

Research Products

• [Journal Article] Calcium Wave Promotes Cell Extrusion (2020)
  • Author(s): Takeuchi Y、Narumi R、Akiyama R、Vitiello E、Shirai T、Tanimura N、Kuromiya K、Ishikawa S、Kajita M、Tada M、Haraoka Y、Akieda Y、 Ishitani T、Fujioka Y、Ohba Y、Yamada S、Hosokawa Y、Toyama Y、Matsui T、Fujita Y
  • Journal Title: Current Biology Volume: 30 Issue: 4 Pages: 670-681.e6
  • DOI: 10.1016/j.cub.2019.11.089
  • NAID: 120006960639
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Noise-resistant developmental reproducibility in vertebrate somite formation (2019)
  • Author(s): Honda N*, Akiyama R, Sari DWK, Ishii S, Bessho Y and Matsui T
  • Journal Title: PLoS Computational Biology Volume: 15(2) Issue: 2 Pages: e1006579-e1006579
  • DOI: 10.1371/journal.pcbi.1006579
  • NAID: 120006553009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ERK Activity Dynamics during Zebrafish Embryonic Development (2018)
  • Author(s): Wong Kah-Loon、Akiyama Ryutaro、Bessho Yasumasa、Matsui Takaaki
  • Journal Title: International Journal of Molecular Sciences Volume: 20 Issue: 1 Pages: 109-109
  • DOI: 10.3390/ijms20010109
  • NAID: 120006555362
  • Peer Reviewed / Open Access
• [Journal Article] Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm. (2018)
  • Author(s): Sari DWK, Akiyama R, Naoki H, Ishijima H, Bessho Y, Matsui T
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 4335-4335
  • DOI: 10.1038/s41598-018-22619-9
  • NAID: 120006555360
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules (2018)
  • Author(s): Tahara Naoyuki、Akiyama Ryutaro、Theisen Joshua W.M.、Kawakami Hiroko、Wong Julia、Garry Daniel J.、Kawakami Yasuhiko
  • Journal Title: Developmental Biology Volume: 434 Issue: 1 Pages: 74-83
  • DOI: 10.1016/j.ydbio.2017.11.013
  • Peer Reviewed