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Mechanism of transcriptional repression by GAF1-GRAS complex

Research Project

Project/Area Number 17K15143
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionHiroshima University

Principal Investigator

Ito Takeshi  広島大学, 理学研究科, 研究員 (30636139)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords転写抑制 / 転写制御 / 遺伝子発現制御 / ジベレリン / DELLA / 植物ホルモン / 植物 / 生体分子 / 蛋白質 / 発現制御
Outline of Final Research Achievements

GAF1-DELLA complex activates the transcription of gibberellin biosynthetic genes. GRX almost completely suppresses the transcriptional activity of GAF1-DELLA. The aim of this study was to clarify the mechanism of transcriptional repression by GRX. GRX repressed the transcriptional activity of GAF1-DELLA complex by the repression activity of GRX itself, but not by competitive inhibition of the interaction between GAF1 and DELLA. GRX repressed the transcription in a TOPLESS-independent manner. The repression activity of GRX was enhanced in the presence of GAF1. GRX exerted the repression activity even in the presence of DELLA. GRX may repress the transcription by forming a ternary complex with GAF1 and DELLA.

Academic Significance and Societal Importance of the Research Achievements

GAの内生量はフィードバック制御により一定の範囲内に保たれている。植物は、環境に応じて適切に成長が制御されており、例えば、乾燥や塩、低温などのストレス条件下では、GA生合成酵素遺伝子の発現が低下し、植物は成長を抑制する。このような状況では、DELLAタンパク質が蓄積するにも関わらず、GA生合成酵素遺伝子の発現の上昇は見られない。したがって、フィードバック制御を一時的に抑制し、GA生合成酵素遺伝子の発現を抑圧する上位の制御が存在すると推定された。本研究により、このような制御の分子メカニズムの一部が明らかになった。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (19 results)

All 2019 2018 2017

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (13 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Autophosphorylation of Ser-6 via an intermolecular mechanism is important for the rapid reduction of NtCDPK1 kinase activity for substrate RSG2018

    • Author(s)
      Ito Takeshi、Ishida Sarahmi、Takahashi Yohsuke
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 4 Pages: e0196357-e0196357

    • DOI

      10.1371/journal.pone.0196357

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] DELLA-dependent and -independent gibberellin signaling2018

    • Author(s)
      Ito, T., Okada, K., Fukazawa, J. and Takahashi, Y
    • Journal Title

      Plant Signal Behav.

      Volume: 13 Issue: 3 Pages: e1445933-e1445933

    • DOI

      10.1080/15592324.2018.1445933

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Subcellular localization of VIP1 is regulated by phosphorylation and 14-3-3 proteins2017

    • Author(s)
      Takeo Koichi、Ito Takeshi
    • Journal Title

      FEBS Letters

      Volume: 591 Issue: 13 Pages: 1972-1981

    • DOI

      10.1002/1873-3468.12686

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Autophosphorylation affects substrate-binding affinity of tobacco Ca2+-dependent protein kinase12017

    • Author(s)
      Ito, T., Ishida, S., Oe, S., Fukazawa, J. and Takahashi, Y.
    • Journal Title

      Plant Physiology

      Volume: 174 Issue: 4 Pages: 2457-2468

    • DOI

      10.1104/pp.17.00515

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] DELLA-GAF1 complex is a main component in gibberellin feedback regulation of GA 20-oxidase 22017

    • Author(s)
      Fukazawa, J., Mori, M., Watanabe, S., Miyamoto, C., Ito, T. and Takahashi, Y.
    • Journal Title

      Plant Physiology

      Volume: 175 Issue: 3 Pages: 1395-1406

    • DOI

      10.1104/pp.17.00282

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gibberellin induces an increase in cytosolic Ca2+ via a DELLA-independent signaling pathway2017

    • Author(s)
      Okada, K., Ito, T., Fukazawa, J. and Takahashi, Y.
    • Journal Title

      Plant Physiology

      Volume: 175 Issue: 4 Pages: 1536-1542

    • DOI

      10.1104/pp.17.01433

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] ジベレリンによる花成制御機構の解析2019

    • Author(s)
      深澤壽太郎, 大橋由紀, 中居可奈子, 髙橋竜平, 伊藤岳, 高橋陽介
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] GAF1とその相互作用因子によるジベレリン生合成酵素遺伝子の転写制御2019

    • Author(s)
      伊藤岳, 勝部隆義, 深澤壽太郎, 高橋陽介
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナにおけるDELLA 複合体によるABA 感受性の制御機構2018

    • Author(s)
      中林誠太朗,伊藤岳,深澤壽太郎,高橋陽介
    • Organizer
      中国四国植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ジベレリンによる花成制御機構の解析2018

    • Author(s)
      大橋由紀, 髙橋竜平, 伊藤岳, 深澤壽太郎, 髙橋陽介
    • Organizer
      中国四国植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カルシウム依存性タンパク質リン酸化酵素NtCDPK1の自己リン酸化による機能制御2018

    • Author(s)
      伊藤岳, 深澤壽太郎, 高橋陽介
    • Organizer
      中国四国植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ジベレリン信号伝達に関与するカルシウム依存性タンパク質リン酸化酵素NtCDPK1の自己リン酸化による機能制御の解析2018

    • Author(s)
      伊藤岳, 石田さらみ, 深澤壽太郎, 高橋陽介
    • Organizer
      日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ジベレリン信号伝達におけるDELLA非依存的な細胞質カルシウムイオンの上昇経路2018

    • Author(s)
      岡田佳那子, 伊藤岳, 深澤壽太郎, 高橋陽介
    • Organizer
      日本植物生理学会(札幌)
    • Related Report
      2017 Research-status Report
  • [Presentation] Autophosphorylation affects substrate-binding affinity of tobacco Ca2+-dependent protein kinase NtCDPK12017

    • Author(s)
      Ito Takeshi、Ishida Sarahmi、Fukazawa Jutarou、Takahashi Yohsuke
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] DELLA-GAF1 complex regulates gibberellin homeostasis and signaling in Arabidopsis2017

    • Author(s)
      Fukazawa Jutarou、Ito Takeshi、Takahashi Yohsuke
    • Organizer
      5th International symposium on Plant Signaling and Behavior 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Autophosphorylation affects substrate-binding affinity of tobacco Ca2+-dependent protein kinase NtCDPK12017

    • Author(s)
      Ito Takeshi、Ishida Sarahmi、Fukazawa Jutarou、Takahashi Yohsuke
    • Organizer
      Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ジベレリンによるシロイヌナズナの花成制御2017

    • Author(s)
      大橋由紀, 髙橋竜平, 伊藤岳, 深澤壽太郎, 高橋陽介
    • Organizer
      中国四国植物学会(高知)
    • Related Report
      2017 Research-status Report
  • [Presentation] GAF1 とその相互作用因子によるジベレリン生合成酵素遺伝子の転写制御2017

    • Author(s)
      勝部隆義, 伊藤岳, 深澤壽太郎, 高橋陽介
    • Organizer
      中国四国植物学会(高知)
    • Related Report
      2017 Research-status Report
  • [Presentation] DELLA を介したジベレリンとジャスモン酸のクロストーク制御2017

    • Author(s)
      森亮太,藤井麻弥,伊藤岳,深澤壽太郎,高橋陽介
    • Organizer
      中国四国植物学会(高知)
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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