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A novel light-dependent behavior of nucleoids in chloroplasts

Research Project

Project/Area Number 17K15145
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionKonan University

Principal Investigator

Iwabuchi Kosei  甲南大学, 理工学部, 研究員 (30583471)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords葉緑体 / 核様体 / 光応答反応 / 光分散 / 暗集合 / 光合成 / ゼニゴケ / 光応答 / ライブイメージング / 光分散反応 / 暗集合反応 / cpDNA / 暗凝集反応 / SiR / DNA / 光 / 凝集 / 分散 / 植物細胞
Outline of Final Research Achievements

We recently found that the chloroplast nucleoids of the liverwort Marchantia polymorpha gather at the center of chloroplasts in the dark and disperse evenly forming small speckles and throughout the chloroplasts under white-light condition. In this study, we investigated the basic features of the nucleoid responses in more detail. We revealed that the nucleoid dispersal depended on light intensities but not on light wavelengths, and on photosynthesis. We further generated transgenic Marchantia plants expressing MpSiR-YFP, a fluorescenet marker of nucleoids, thereby allowing the live imaging of nucleoids.This study provides an oppotunity to understand the chloroplast functions under flucuating light conditions from the viewpoint of nucleoid dynamics .

Academic Significance and Societal Importance of the Research Achievements

本研究では葉緑体内部で起こる光応答反応に着目し、核様体の空間的配置という独自の視点で葉緑体機能を理解する。本研究が達成されれば植物の新たな光応答反応が明らかになる。これは基礎生物学や光生物学分野において極めて重要な発見であると考えている。また、本研究において核様体の分布と光合成との関連が見えてきた。光合成の調節機構を完全に解き明かすことは、人工光合成研究への応用だけでなく、植物の光合成能力の向上や今後変動しうる自然環境の中で植物が生き残るための機能を知る上でも重要である。本研究はこうした応用研究を支える重要な基礎研究と考え、更なる研究を展開していく。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (10 results)

All 2020 2019 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results) Remarks (2 results)

  • [Journal Article] A space-saving visual screening method, Glycine max FAST, for generating transgenic soybean2020

    • Author(s)
      Iwabuchi Kosei、Shimada Takashi L.、Yamada Tetsuya、Hara-Nishimura Ikuko
    • Journal Title

      Plant Signaling & Behavior

      Volume: 15 Issue: 2 Pages: 1722911-1722911

    • DOI

      10.1080/15592324.2020.1722911

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] tRNA Wobble Modification Affects Leaf Cell Development in Arabidopsis thaliana2019

    • Author(s)
      Nakai Yumi、Horiguchi Gorou、Iwabuchi Kosei、Harada Akiko、Nakai Masato、Hara-Nishimura Ikuko、Yano Takato
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 9 Pages: 2026-2039

    • DOI

      10.1093/pcp/pcz064

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physiological function of photoreceptor UVR8 in UV-B tolerance in the liverwort Marchantia polymorpha2019

    • Author(s)
      Kondou Youichi、Miyagi Yuta、Morito Takeshi、Fujihira Kenta、Miyauchi Wataru、Moriyama Asami、Terasawa Takuya、Ishida Sakiko、Iwabuchi Kosei、Kubo Hiroyoshi、Nishihama Ryuichi、Ishizaki Kimitsune、Kohchi Takayuki
    • Journal Title

      Planta

      Volume: 249 Issue: 5 Pages: 1349-1364

    • DOI

      10.1007/s00425-019-03090-w

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 薬剤選抜を必要としない迅速な形質転換ダイズの作製法(GmFAST法)2020

    • Author(s)
      岩渕功誠、島田貴士、山田哲也、西村いくこ
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 葉緑体核様体の新奇光応答反応-光分散・暗集合反応-2019

    • Author(s)
      石原静圭、坂下幸汰、石田悠介、木森義隆、西村芳樹、小林優介、西村いくこ、岩渕功誠
    • Organizer
      近畿植物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 薬剤選抜を必要としない迅速な形質転換ダイズの作製法(GmFAST法)2019

    • Author(s)
      岩渕功誠、島田貴士、山田哲也、西村いくこ
    • Organizer
      近畿植物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 葉緑体核様体の光分散・暗凝集反応2019

    • Author(s)
      岩渕功誠
    • Organizer
      エンドメンブレンミーティング
    • Related Report
      2019 Annual Research Report
  • [Presentation] 葉緑体核様体に見られる新奇光応答反応2019

    • Author(s)
      石原静圭,坂下幸汰,石田悠介,木森義隆,岩渕功誠,西村いくこ
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Research-status Report
  • [Remarks] researchmap

    • URL

      https://researchmap.jp/iwabuchikosei

    • Related Report
      2019 Annual Research Report
  • [Remarks] 甲南大・理工・植物細胞生物学研究室

    • URL

      https://sites.google.com/view/pumpkin-pumpkin/%E3%83%9B%E3%83%BC%E3%83%A0

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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