• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of a mechanism of heterocyst differentiation in a multicellular cyanobacterium by measuring Raman spectra

Research Project

Project/Area Number 17K15151
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionChiba University

Principal Investigator

Ishihara Jun-ichi  千葉大学, 真菌医学研究センター, 特任助教 (40732409)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsラマン散乱分光 / ラマンスペクトル / シアノバクテリア / ヘテロシスト / 光合成色素 / 微生物
Outline of Final Research Achievements

I studied about “how heterocyst cells are differentiated at almost every 10 cells along a filament in a multicellular cyanobacterium”. First, I measured Raman spectra from vegetative and heterocyst cells, and found that Raman bands were assigned to four kinds of photosynthesis proteins. Especially, the Raman bands assigned to phycocyanin were gradually decreased during the heterocyst differentiation. Second, such changes in the Raman band intensities were observed in adjacent several vegetative cells. When any one cell was selected to be differentiated, the Raman bands assigned to phycocyanin were reversely increased in other cells. That is, we showed that the cellular fate is determined thorough the cellular interaction by measuring the Raman bands assigned to the photosynthesis proteins.

Academic Significance and Societal Importance of the Research Achievements

細胞内の生体分子の濃度や局在を計測するとき、例えば蛍光タンパク質などで標識化する方法が主流である。しかし、この方法を用いた場合、その分子の機能が失われたり、また合成や分解を時間差なくトレースすることが難しい。本研究では、ラマンスぺクトル計測がこれらの問題点を克服し、標的分子のダイナミクスを非標識かつリアルタイムで解析できることを示した。さらに、ラマンスペクトルには多数の分子に帰属されるラマンバンドが含まれるため、1回の計測でこれらの分子のダイナミクスを同時に得ることができる。したがって、研究代表者は、ラマンスペクトル解析が生命科学の研究に有効であり、次世代の手法になりうる可能性を示した。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] DIpartite: A tool for detecting bipartite motifs by considering base interdependencies2019

    • Author(s)
      Mohammad Vahed, Jun-ichi Ishihara, Hiroki Takahashi
    • Journal Title

      PLOS ONE

      Volume: 14(8): e0220207 Issue: 8 Pages: 1-14

    • DOI

      10.1371/journal.pone.0220207

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Mathematical Analysis of Copper Efflux system in Escherichia coli2019

    • Author(s)
      Jun-ichi Ishihara, Tomohiro Mekubo, Chikako Kusaka, Suguru Kondou, Naotake Ogasawara, Taku Oshima, Hiroki Takahashi
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Raman Microscopic Analysis of Differentiation Process of Multi-Cellular Cyanobacterium2017

    • Author(s)
      石原潤一、高根沢聡太、岩崎秀雄、盛田伸一
    • Organizer
      日本分光学会年次講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] Discussion about Pretreatments of Bio-Raman Spectra2017

    • Author(s)
      盛田伸一、石原潤一
    • Organizer
      日本分光学会年次講演会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi