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Functional analysis of sex differentiation by Piwi-piRNA system expressed in germ cells

Research Project

Project/Area Number 17K15152
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionOsaka Medical College

Principal Investigator

Yamamoto Yasuhiro  大阪医科大学, 医学部, 講師 (20613558)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords性分化 / PIWI-piRNA / 生殖
Outline of Final Research Achievements

To understand the role of PIWI-piRNA system in medaka sex differentiation, we analyzed the PIWI mutant medaka and whole genome analysis of small RNAs. Some Piwil1 mutants showed no secondary sex characteristics and predominantly low sex hormone levels. The number of ftz-f1 positive cells was decreased in the gonads of Piwil1 mutants, and expression of sexual differentiation-related genes was not confirmed in the gonads of Piwil1 mutants. In NGS analysis of small RNAs in gametes, we identified a candidate genes of Piwil1 target whose expression was elevated only in germ cells of the Piwil1 mutant. From these results, it was expected that the Piwil1 mutant could not suppress the expression of the target genes, leading to the failure of sexual differentiation in the gonad.

Academic Significance and Societal Importance of the Research Achievements

カイコや柿ではPiwi-piRNAによる性分化制御機構が明らかになっている。魚類やほ乳類においてもPIWIの変異体の表現型解析から、性分化におけるPiwi-piRNAの役割が示唆されてきた。本研究により、生殖細胞で特異的に発現するpiRNAが生殖腺体細胞のステロイド産生を制御しており、個体の二次性徴に関わることを示した。本研究は「piRNAが標的遺伝子の発現制御を介して、性ホルモンを調節する」全く新しい知見と考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2020 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] Gastrointestinal Neurons Expressing HCN4 Regulate Retrograde Peristalsis2020

    • Author(s)
      Fujii Kensuke、Nakajo Koichi、Egashira Yoshihiro、Yamamoto Yasuhiro、Kitada Kazuya、Taniguchi Kohei、Kawai Masaru、Tomiyama Hideki、Kawakami Koichi、Uchiyama Kazuhisa、Ono Fumihito
    • Journal Title

      Cell Reports

      Volume: 30 Issue: 9 Pages: 2879-2888

    • DOI

      10.1016/j.celrep.2020.02.024

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synaptic silencing of fast muscle is compensated by rewired innervation of slow muscle2020

    • Author(s)
      Zempo B, Yamamoto, Y., Williams, T., Ono
    • Journal Title

      Science Advances

      Volume: 6 Pages: 1-12

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] トランスポゾンの抑制におけるメダカPIWIの役割2018

    • Author(s)
      山本耕裕
    • Organizer
      日本動物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] メダカ配偶子において雌雄差を示すpiRNAの網羅的同定2017

    • Author(s)
      山本耕裕・大竹規仁・齋藤大介・西村俊哉・田中実
    • Organizer
      日本動物学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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