Identification of physiological function of reverse photoreceptor, Opn5L1
| Project/Area Number |
17K15159
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Okayama University |
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Principal Investigator |
Sato Keita 岡山大学, 医歯薬学総合研究科, 助教 (80725622)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 光受容 / 非視覚性光受容 / オプシン / 非視覚光受容 / メダカ / ピノプシン / 生理学 / 神経科学 |
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| Outline of Final Research Achievements |
To clarify the physiological function of reverse photoreceptor Opn5L1, an attempt was made to develop a transgenic medaka fish which expresses a reporter gene such as wheat germ agglutinin, green fluorescent protein, or nitroreductase specifically in the Opn5L1-positive cells. For development of a transgenic medaka, the two plasmid vectors were constructed. One carries CRE recombinase under the Opn5L1 promoter and green fluorescent protein under the cardiac muscle-specific promoter. The other carries reporter gene with the unpstream floxed transcription stop cassette under beta-actin promoter and red fluorescent protein under the cardiac muscle-specific promoter. Additionally, the apparatus which semi-automatically records and analyzes the behavior of transgenic medaka fishes under various lighting conditions was constructed.
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| Academic Significance and Societal Importance of the Research Achievements |
光が動物の生理機能におよぼす影響についての理解は、太陽光、月光、あるいは生物発光や人工的な光など様々な光源が存在するこの世界にヒトを含む動物がどのように適応し、生活しているかを理解するために重要である。形・色・動きを捉えるための光受容システムである視覚は非常によく研究が進んでいるが、非視覚性の光受容システムに関してはまだまだ知見が少ない。
本研究の成果をもとにトランスジェニック動物を作製し、解析を進めることによって、非視覚性の光受容に関する理解が進み、動物がどのように光を利用するかについて理解が広がると期待される。
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Pinopsin evolved as the ancestral dim-light visual opsin in vertebrates2018
Author(s)
Keita Sato, Takahiro Yamashita, Keiichi Kojima, Kazumi Sakai, Yuki Matsutani, Masataka Yanagawa, Yumiko Yamano, Akimori Wada, Naoyuki Iwabe, Hideyo Ohuchi, Yoshinori Shichida
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Journal Title
Communications Biology
Volume: 1
Issue: 1
Pages: 156-156
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Vertebrate photoreceptor, Opn5L1, is the newcomer of opsin acting as a reverse and self-regenerating photoreceptor2018
Author(s)
Keita Sato, Takahiro Yamashita, Hideyo Ohuchi, Atsuko Takeuchi, Hitoshi Gotoh, Katsuhiko Ono, Misao Mizuno, Yasuhisa Mizutani, Sayuri Tomonari, Kasumi Sakai, Yasushi Imamoto, Akimori Wada, Yoshinori Shichida
Organizer
第56回日本生物物理学会年会
Related Report
Invited
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