Investigation of interspecific differences in regulatory mechanism of photosynthesis by spectroscopic imaging and microclimate analysis

• Project/Area Number: 17K15192
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Nagoya University
• Principal Investigator: Sugiura Daisuke 名古屋大学, 生命農学研究科, 助教 (50713913)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 微気象 / 光合成 / イネ / 葉面積指数 / 近赤外光 / 光合成有効放射 / 分光計測 / 非構造性炭水化物 / ハイパースペクトル / クロロフィル蛍光

Outline of Final Research Achievements

This study aimed at elucidating responses of photosynthetic characteristics and canopy growth to micro-climate and their interspecific or intercultivar differences in crops. Physiological mechanisms of down-regulation of photosynthesis responding to non-structural carbohydrates in soybean and French bean were closely examined. We also developed a technique to estimate rice canopy growth non-destructively by measuring near infra-red radiation (NIR) and photosynthetically active radiation (PAR) inside and outside rice canopy. This technique allowed us to trace seasonal dynamics of canopy growth from transplanting to maturity stage and to evaluate varietal and regional differences in the canopy growth characteristics.

Academic Significance and Societal Importance of the Research Achievements

植物の物質生産の基盤である光合成の調節機構の生理メカニズムや種間差、群落レベルの成長量の非破壊測定法を確立できたことで、野外環境における微気象が植物の光合成や成長に与える影響を容易に評価できるようになった。これらの研究成果は、将来環境において、大気CO2濃度の上昇や温暖化が作物の生産性に与える影響を予測することに役立つことが期待される。

Research Products

• [Journal Article] Elevated CO2-induced changes in mesophyll conductance and anatomical traits in wild type and carbohydrate-metabolism mutants of Arabidopsis. (2019)
  • Author(s): Mizokami Y, Sugiura D, Watanabe CKA, Betsuyaku E, Inada N, Terashima I
  • Journal Title: J. Exp. Bot. Volume: 70 Issue: 18 Pages: 4807-4818
  • DOI: 10.1093/jxb/erz208
  • Peer Reviewed / Open Access
• [Journal Article] Interspecific differences in how sink-source imbalance causes photosynthetic downregulation among three legume species. (2019)
  • Author(s): Sugiura D, Betsuyaku E, Terashima I.
  • Journal Title: Annals of Botany Volume: 123 Issue: 4 Pages: 715-726
  • DOI: 10.1093/aob/mcy204
  • Peer Reviewed / Open Access
• [Journal Article] Shoot nitrate underlies a perception of nitrogen satiety to trigger local and systemic signaling cascades in Arabidopsis thaliana. (2019)
  • Author(s): Okamoto Y, Suzuki T, Sugiura D, Kiba T, Sakakibara H, Hachiya T.
  • Journal Title: Soil science and plant nutrition Volume: 65 Issue: 1 Pages: 56-64
  • DOI: 10.1080/00380768.2018.1537643
  • NAID: 210000012050
  • Peer Reviewed / Open Access
• [Journal Article] Effects of elevated atmospheric CO2 on respiratory rates in mature leaves of two rice cultivars grown at a free-air CO2 enrichment site and analyses of the underlying mechanisms. (2018)
  • Author(s): Ko Noguchi, Tomonori Tsunoda, Atsuko Miyagi, Maki Kawai-Yamada, Daisuke Sugiura, Shin-Ichi Miyazawa, Takeshi Tokida, Yasuhiro Usui, Hirofumi Nakamura, Hidemitsu Sakai, Toshihiro Hasegawa
  • Journal Title: Plant and Cell Physiology Volume: 59 Issue: 3 Pages: 637-649
  • DOI: 10.1093/pcp/pcy017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sink-Source Balance and Down-Regulation of Photosynthesis in Raphanus sativus: Effects of Grafting, N and CO2 (2017)
  • Author(s): Sugiura Daisuke, Watanabe Chihiro K. A., Betsuyaku Eriko, Terashima Ichiro
  • Journal Title: Plant & cell physiology Volume: 58 Issue: 12 Pages: 2043-2056
  • DOI: 10.1093/pcp/pcx132
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Arabidopsis Phosphatidic Acid Phosphohydrolases Are Essential for Growth under Nitrogen-Depleted Conditions (2017)
  • Author(s): Yoshitake Yushi, Sato Ryoichi, Madoka Yuka, Ikeda Keiko, Murakawa Masato, Suruga Ko, Sugiura Daisuke, Noguchi Ko, Ohta Hiroyuki, Shimojima Mie
  • Journal Title: Frontiers in Plant Science Volume: 8 Pages: 1-11
  • DOI: 10.3389/fpls.2017.01847
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 近赤外光・光合成有効放射比の連続測定によるイネ群落の非破壊LAI計測法の開発 (2019)
  • Author(s): 杉浦大輔, 福田将大, 細井淳, 近藤始彦
  • Organizer: 日本作物学会第247回講演会
• [Presentation] 非破壊的LAI計測法を応用したイネ群落成長の微気象応答の品種間・地域間差の解明 (2019)
  • Author(s): 福田将大, 杉浦大輔
  • Organizer: 日本生態学会第66回全国大会
• [Presentation] イネ品種における内生窒素固定能と非構造性炭水化物含量の関係 (2018)
  • Author(s): 岡本 卓哲, 新庄 莉奈, 田中 愛子, 杉浦 大輔, 近藤 始彦
  • Organizer: 日本作物学会第245回講演会
• [Presentation] Genome-Wide Analysis of Gene Expression Regulated by Internal Nitrate in Arabidopsis Thaliana (2017)
  • Author(s): Yuki Okamoto, Mikiko Kojima, Yumiko Takebayashi, Daisuke Sugiura, Takamasa Suzuki, Hitoshi Sakakibara, Takushi Hachiya
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research
• [Presentation] ダイズとインゲンにみる光合成ダウン/アップレギュレーションの生理生態学的意義 (2017)
  • Author(s): 杉浦 大輔, 河野 優, 矢守 航, 寺島 一郎
  • Organizer: 日本作物学会第244回講演会
• [Presentation] 体内の硝酸イオンと同化窒素を一方または両方欠乏させた植物体における遺伝子発現応答の解析 (2017)
  • Author(s): 岡本悠希, 杉浦大輔, 鈴木孝征, 榊原均, 蜂谷卓士
  • Organizer: 日本土壌肥料学会2017年度仙台大会