| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
The blue-light response, independent of leaf photosynthesis, is a critical response on regulating stomatal opening in many C3 plant. However, the applicant found that in C4 plant, the stomata probably lack this blue-light response. This research proposal is planned to investigate this novel phenomenon in details, and explore possible reasons why do stomata of C4 plant lack blue-light response.
In this study, physiological and biosynthetic analysis of stomatal opening reaction defects dependent on blue light in C4 plants was carried out. Monocotyledonous and dicotyledonous leaves and three subtypes of C4 plant including Arabidopsis, rice, corn, sorghum, guinea grass, Amaranthus, C3 or C4 species each of the Flaveria genus were used in this study. By performing an experimental method such as immunostaining of the gas exchange system and isolated epidermis or guard cells, the main causes of stomatal opening reaction defect dependent on blue light were analyzed in detailed.
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