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Mechanisms of endosperm development controlled by Polycomb repressive complex 2 in rice

Research Project

Project/Area Number 17K15210
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Research InstitutionIwate University (2019)
Yokohama City University (2017-2018)

Principal Investigator

Tonosaki Kaoru  岩手大学, 農学部, 助教 (20749494)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsイネ / 胚乳 / ポリコーム複合体 / 胚乳発生 / 自律的胚乳発生 / インプリント遺伝子 / 育種学 / ポリコーム
Outline of Final Research Achievements

In this study, we demonstrate that mutation of the rice gene OsEMF2a, which encodes a component of PRC2, causes an abnormally delayed developmental transition in the endosperm. In addition, transcriptome and H3K27me3 ChIP-seq analyses in Osemf2a mutant identified the putative downstream targets of PRC2, that are required for endosperm development. Before fertilization, we observed an autonomous endosperm phenotype in Osemf2a mutant, even in the absence of fertilization. Detailed histological and transcriptomic analyses revealed that the autonomous endosperm can produce storage compounds, starch granules and protein bodies specific to the endosperm.

Academic Significance and Societal Importance of the Research Achievements

イネの胚乳は、可食部となる器官であり、その大きさや品質が商品価値を決定する主要因であるため、胚乳の発生機構の理解は農作物としてのイネの重要な課題である。そのため、胚乳発生制御を担うPRC2の機能とその標的遺伝子を明らかにできたことで、胚乳発生制御ネットワークの理解が深まると期待される。また、イネの胚乳におけるPRC2の機能に関する報告は本研究が初めてであり、PRC2の機能が広範な植物種に保存されていることを示すことができた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) Remarks (2 results)

  • [Journal Article] Endosperm Development2018

    • Author(s)
      Kaoru Tonosaki and Tetsu Kinoshita
    • Journal Title

      eLS

      Volume: - Pages: 1-7

    • DOI

      10.1002/9780470015902.a0020098.pub2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overcoming the species barrier by ploidy manipulation in the genus Oryza2018

    • Author(s)
      Tonosaki Kaoru、Sekine Daisuke、Ohnishi Takayuki、Ono Akemi、Furuumi Hiroyasu、Kurata Nori、Kinoshita Tetsu
    • Journal Title

      The Plant Journal

      Volume: 93 Issue: 3 Pages: 534-544

    • DOI

      10.1111/tpj.13803

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 特集記事 ワークショップ報告「受精後雑種障壁研究の育種利用に向けて」2017

    • Author(s)
      石井孝佳・岡本龍史・殿崎 薫・長岐清孝・木下 哲
    • Journal Title

      育種学研究

      Volume: 19 Pages: 35-40

    • Related Report
      2017 Research-status Report
    • Open Access
  • [Presentation] 自律的胚乳発生を示すOsEMF2aの形態学的解析2019

    • Author(s)
      国定愛美, 殿崎薫, 西野愛, 小野明美, 木下哲
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネ胚乳発生におけるOsEMF2aの機能解析2019

    • Author(s)
      西野愛、殿崎薫、国定愛美、小野明美、木下哲
    • Organizer
      日本育種学会 第135回講演会
    • Related Report
      2018 Research-status Report
  • [Remarks] research map

    • URL

      https://researchmap.jp/ktonosaki

    • Related Report
      2018 Research-status Report
  • [Remarks] research gate

    • URL

      https://www.researchgate.net/profile/Kaoru_Tonosaki

    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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