Establishment of analysis method for double fertilization process using unreduced male gamete
Project/Area Number |
17K15223
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Horticultural science
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Research Institution | University of Miyazaki |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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Keywords | 雄性配偶子 / 花粉 / 重イオンビーム / 重複受精 / イオンビーム / 精細胞 / 雄原細胞 / キルタンサス |
Outline of Final Research Achievements |
We have successfully induced unique male gametes in Cyrtanthus mackenii by using heavy-ion beam irradiation; the sperm cell formation is prevented and the generative cells are not divided into two sperm cells. The male gametes are called generative-cell-like sperm cells (GC-like SCs). In this study, we analyzed embryogenesis after pollination of the irradiated pollen grains to reveal behavior of the GC-like SCs during the double fertilization. When the irradiated pollen grains were crossed, few embryo sacs contained endosperm and undivided egg cell. From the results by nuclear DNA fluoresce analysis in the abnormal embryo sac, it is suggested that the abnormal embryo sacs were formed by fusing GC-like SC with polar nuclei. This phenomenon will be utilized for ornamental plant breeding.
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Academic Significance and Societal Importance of the Research Achievements |
本研究により、多様な倍数性を有する胚および胚乳が得られたことから、今後胚珠培養や胚培養、胚乳培養を行うことで、倍数性の多様化法として雄原細胞様精細が利用できる可能性が示された。キルタンサスのような二細胞性花粉を持つ園芸作物では、雄原細胞様精細胞が誘発可能と考えられることから、新たな育種法として迅速な染色体添加、染色体欠失系統の作出や倍数性育種への応用が期待される。
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Report
(4 results)
Research Products
(7 results)