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Analysis on postzygotic isolation mechanisms in interspecific crosses of Capsicum

Research Project

Project/Area Number 17K15224
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionOsaka Prefecture University

Principal Investigator

Tezuka Takahiro  大阪府立大学, 生命環境科学研究科, 講師 (20508808)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords雑種弱勢 / 温度感受性 / 細胞死 / 糖 / 病害抵抗性 / rDNA-ITS配列 / SSRマーカー / トウガラシ / 種間交雑 / 生殖隔離 / 系統解析 / 植物遺伝育種 / 種分化 / ITS配列 / ピーマン / 座止 / 発育不全
Outline of Final Research Achievements

In this study, hybrid weakness, a type of postzygotic reproductive isolation, in the genus Capsicum was analyzed. The hybrid weakness phenotype was evidenced by almost complete arrest of new leaf formation, delayed increase in plant height, and reduced upper internode length. The shoot apical meristem (SAM) of F1 plants exhibited delayed development and an abnormal structure characterized by a flat shape and the presence of fuzzy cell layers on the surface. Hybrid weakness was temperature sensitive and suppressed at elevated temperatures. It was revealed that disease resistance-related genes and programmed cell death were related to hybrid weakness, and sugars were accumulated in hybrid plants. Furthermore, allelic distribution of hybrid weakness genes was investigated using molecular phylogenetic analyses and test crosses.

Academic Significance and Societal Importance of the Research Achievements

遠縁交雑育種は、遺伝子組換えを行わずに、野生種等から栽培種に有用形質を導入できる育種法である。しかし、遠縁の植物種間では、種の独立性を維持するための機構である生殖隔離がしばしば認められ、遠縁交雑によるF1雑種作出やその後代の育成が困難あるいは不可能であることが多い。そのため、生殖隔離を回避・克服する技術が求められている。本研究では、トウガラシの種間交雑で認められる雑種弱勢(生殖隔離)を解析し、どのような表現型の異常が生じているのか、どのような遺伝子が関与しているのか、などを明らかにした。一方で、雑種植物を高温で栽培することで雑種弱勢を抑制できることも明らかにした。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (24 results)

All 2020 2019 2018 Other

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (13 results) (of which Int'l Joint Research: 4 results) Remarks (4 results)

  • [Journal Article] Phenylalanine ammonia-lyase and phenolic compounds are related to hybrid lethality in the cross <i>Nicotiana suaveolens</i>×<i>N. tabacum</i>2020

    • Author(s)
      Shiragaki Kumpei、Nakamura Rie、Nomura Shigeki、He Hai、Yamada Tetsuya、Marubashi Wataru、Oda Masayuki、Tezuka Takahiro*
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 3 Pages: 327-333

    • DOI

      10.5511/plantbiotechnology.20.0606a

    • NAID

      130007907497

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2020-09-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A hypersensitive response-like reaction is involved in hybrid dwarfism in F1 plants of the cross Capsicum annuum × Capsicum chinense.2020

    • Author(s)
      Kumpei Shiragaki, Shuji Yokoi and Takahiro Tezuka
    • Journal Title

      Breeding Science

      Volume: 70 Issue: 4 Pages: 430-437

    • DOI

      10.1270/jsbbs.19137

    • NAID

      130007895837

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phylogenetic Analysis and Molecular Diversity of Capsicum Based on rDNA-ITS Region2020

    • Author(s)
      Shiragaki Kumpei、Yokoi Shuji、Tezuka Takahiro*
    • Journal Title

      Horticulturae

      Volume: 6 Issue: 4 Pages: 87-87

    • DOI

      10.3390/horticulturae6040087

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A hypersensitive response-like reaction is involved in hybrid weakness in F1 plants of the cross Capsicum annuum × Capsicum chinense2020

    • Author(s)
      Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka*
    • Journal Title

      Breeding Science

    • NAID

      130007895837

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Toward the elucidation of reproductive isolation mechanisms in plants: diversity and universality2019

    • Author(s)
      手塚孝弘*・一谷勝之・松本雄一・何海・木下哲・宅見薫雄・久保山勉
    • Journal Title

      Breeding Research

      Volume: 21 Issue: 1 Pages: 75-80

    • DOI

      10.1270/jsbbr.21.W05

    • NAID

      130007664807

    • ISSN
      1344-7629, 1348-1290
    • Year and Date
      2019-06-01
    • Related Report
      2019 Research-status Report
  • [Journal Article] Nicotiana suaveolens accessions with different ploidy levels exhibit different reproductive isolation mechanisms in interspecific crosses with Nicotiana tabacum2019

    • Author(s)
      Hai He, Takahiro Iizuka, Maho Maekawa, Kumi Sadahisa, Toshinobu Morikawa, Masanori Yanase, Shuji Yokoi, Masayuki Oda, Takahiro Tezuka*
    • Journal Title

      Journal of Plant Research

      Volume: 132 Issue: 4 Pages: 461-471

    • DOI

      10.1007/s10265-019-01114-w

    • NAID

      40021952241

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] HWA1- and HWA2-mediated hybrid weakness in rice involves cell death, reactive oxygen species accumulation, and disease resistance-related gene upregulation2019

    • Author(s)
      Kumpei Shiragaki, Takahiro Iizuka, Katsuyuki Ichitani, Tsutomu Kuboyama, Toshinobu Morikawa, Masayuki Oda, Takahiro Tezuka*
    • Journal Title

      Plants

      Volume: 8 Issue: 11 Pages: 450-450

    • DOI

      10.3390/plants8110450

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] トウガラシ雑種弱勢における系統地理学的解析2020

    • Author(s)
      白柿薫平・横井修司・手塚孝弘
    • Organizer
      日本進化学会第22回オンライン大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] トウガラシ属種間交雑で生じる雑種弱勢における温度感受性および糖蓄積2020

    • Author(s)
      白柿薫平・古川一・横井修司・手塚孝弘
    • Organizer
      日本育種学会第138回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] トウガラシ属種間交雑で生じる雑種弱勢の表現型解析2020

    • Author(s)
      白柿薫平・横井修司・手塚孝弘
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 葉緑体DNAを用いたNicotiana suaveolens 8系統の系統解析2020

    • Author(s)
      定久公美・何海・横井修司・手塚孝弘
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] トウガラシ栽培種におけるSSRマーカーを用いた系統解析および雑種弱勢原因遺伝子の分布の調査2020

    • Author(s)
      立田智輝・横井修司・手塚孝弘
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] タバコ種間交雑における子房落下現象には母親の過剰な高次倍数性および種子発育不全が関与する2019

    • Author(s)
      何海・横井修司・手塚孝弘
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 形態形質とrDNAのITS領域を用いたトウガラシ属栽培種5種の系統解析2018

    • Author(s)
      白柿薫平・横井修司・手塚孝弘
    • Organizer
      日本育種学会第134回講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] タバコの異倍数性種間交雑後の子房落下現象における脱離関連遺伝子の発現解析2018

    • Author(s)
      何海・横井修司・手塚孝弘
    • Organizer
      日本育種学会第134回講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] タバコの異倍数性種間交雑に認められる子房落下および種子発育不全2018

    • Author(s)
      手塚孝弘・何海
    • Organizer
      日本育種学会第134回講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] Genetic mapping of the HLA1 locus causing hybrid lethality in tobacco interspecific hybrids2018

    • Author(s)
      Takahiro Tezuka, Sae Imagawa, Akira Hasegawa, Hai He, Shuji Yokoi
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Hybrid dwarfism observed in the cross between Capsicum annuum and C. chinense2018

    • Author(s)
      Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparative analysis of different types of reproductive isolation observed in interspecific-interploidy crosses between two Nicotiana suaveolens accessions and N. tabacum2018

    • Author(s)
      Hai He, Shuji Yokoi, Takahiro Tezuka
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Distinct mechanisms are involved in seed abortion and ovary abscission in interploidy-interspecific crosses between Nicotiana suaveolens and N. tabacum2018

    • Author(s)
      Hai He, Shuji Yokoi, Takahiro Tezuka
    • Organizer
      6th Plant Dormancy Symposium 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] 大阪府立大学大学院 植物育種繁殖学

    • URL

      https://shyokoi.wixsite.com/plantbreeding

    • Related Report
      2020 Annual Research Report
  • [Remarks] 大阪府立大学 植物育種繁殖学研究グループ

    • URL

      https://shyokoi.wixsite.com/plantbreeding

    • Related Report
      2019 Research-status Report 2018 Research-status Report
  • [Remarks] 大阪府立大学大学院 生命環境科学研究科 応用生命科学専攻 植物バイオサイエンス

    • URL

      http://www.biosci.osakafu-u.ac.jp/plantbio/

    • Related Report
      2019 Research-status Report
  • [Remarks] 大阪府立大学 植物育種繁殖学研究グループ

    • URL

      https://shyokoi.wixsite.com/plantbreeding

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

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