| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Anthocyanins which are reddish pigments accumulating in plants do not generally accumulate in parenchyma cells of petals. Accumulation of anthocyanins in parenchyma cells of petals might be one of the ways to alter flower colors. The purpose of this study was the identification of genes determining the ability of anthocyanin accumulation in each cell layer of petals. Some carnation cultivars whose petals did not accumulate anthocyanin not only in parenchyma cells but also abaxial epidermal cells of petals were used. These cultivars also had white margins in each petal. By the RNA-seq between the white margins and reddish central parts of petals, six genes whose expression tendencies were consistent with accumulation of anthocyanins were found. As a result of the expression analysis of these six genes in each cell layer of petals isolated with laser microdissection, it was suggested that a novel bHLH transcription factor regulated the accumulation of anthocyanins in each cell layer.
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