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A combination of radiotracer/electronode/omics methods for the analysis of mineral uptake

Research Project

Project/Area Number 17K15236
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

Kobayashi Natsuko  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (60708345)

Research Collaborator TANOI Keitaro  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsマグネシウム欠乏 / ナトリウム応答 / ミネラル吸収 / 環境応答 / 植物 / 根 / イオン吸収 / マグネシウム / ナトリウム / 放射線 / 栄養学 / 生理学
Outline of Final Research Achievements

Modulation of mineral uptake manner in root is one of the stress response mechanisms. Aiming to characterize the molecular mechanisms underlying the stress response, alteration in ion flux in root was analyzed. In the magnesium starved root showing the maximum magnesium uptake rate, any changes in the expression of the known magnesium transporter genes were found. This result indicates that some molecules yet to be identified can participate in the magnesium uptake under magnesium deficient condition. Addition of 30 mM NaCl caused a temporal upregulation of sodium influx particularly in the mature root, followed by the immediate reduction within several minutes. Sodium influx is suggested to be controlled independently from gene expression regulation at the early stage of salt stress response.

Academic Significance and Societal Importance of the Research Achievements

植物のイオン吸収速度を精度高く解析するためには、均質な根を持つサンプルを準備することが重要で、側根が発生していない幼植物が使いやすい。本研究ではシロイヌナズナの1週齢個体を実験に供し、安定したデータを取得できる実験系を確立することができた。この系は他の実験にも応用できるものである。また、同一栽培系の中で複数の無機元素吸収を解析したことで、元素ごとの吸収の特徴が示された。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] ブリュッセル自由大学(ベルギー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ブリュッセル自由大学(ベルギー)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Magnesium uptake characteristics in Arabidopsis revealed by 28Mg tracer studies2018

    • Author(s)
      Ogura Takaaki、Kobayashi Natsuko I.、Suzuki Hisashi、Iwata Ren、Nakanishi Tomoko M.、Tanoi Keitaro
    • Journal Title

      Planta

      Volume: 248 Issue: 3 Pages: 745-750

    • DOI

      10.1007/s00425-018-2936-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Magnesium deficiency damages the youngest mature leaf in rice through tissue-specific iron toxicity2018

    • Author(s)
      Kobayashi Natsuko I.、Ogura Takaaki、Takagi Keiko、Sugita Ryohei、Suzuki Hisashi、Iwata Ren、Nakanishi Tomoko M.、Tanoi Keitaro
    • Journal Title

      Plant and Soil

      Volume: 428 Issue: 1-2 Pages: 137-152

    • DOI

      10.1007/s11104-018-3658-x

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Magnesium deficiency damages the youngest mature leaf in rice through tissue-specific iron toxicity2018

    • Author(s)
      Natsuko I. KOBAYASHI, Takaaki OGURA, Keiko TAKAGI, Ryohei SUGITA, Hisashi SUZUKI, Ren IWATA, Tomoko M. NAKANISHI, Keitaro TANOI
    • Journal Title

      Plant and Soil

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 葉におけるマグネシウム欠乏症発症メカニズムの生理学的考察2018

    • Author(s)
      小林奈通子、小倉尚晃、小川隆宏、杉田亮平、田野井慶太朗
    • Organizer
      日本土壌肥料学会2017年仙台大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Magnesium Deficiency Damages the Young Mature Leaf Through the Unstabilization of Iron Homeostasis in Rice2018

    • Author(s)
      Natsuko I. Kobayashi, Ryohei Sugita, Taka-aki Ogura, Hisashi Suzuki, Ren Iwata, Tomoko M. Nakanishi, Keitaro Tanoi
    • Organizer
      18th International Plant Nutrition Colloquium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Magnesium Deficiency Damages the Young Mature Leaf Through the Unstabilization of Iron Homeostasis in Rice2017

    • Author(s)
      Natsuko I. Kobayashi, Ryohei Sugita, Taka-aki Ogura, Hisashi Suzuki, Ren Iwata, Tomoko M. Nakanishi, Keitaro Tanoi
    • Organizer
      International Plant Nutrition Colloquium 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 放射性トレーサー28Mgを用いたMg欠乏シロイヌナズナにおけるMg吸収能評価2017

    • Author(s)
      小倉尚晃、小林奈通子、鈴木寿、岩田錬、中西友子、田野井慶太朗
    • Organizer
      日本土壌肥料学会 仙台大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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