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Mechanism of acidic soil tolerance in buckwheat

Research Project

Project/Area Number 17K15239
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

Yokosho Kengo  岡山大学, 資源植物科学研究所, 助教 (50790622)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsソバ / 酸性土壌 / アルミニウム / マンガン / 輸送体 / Nramp / 酸性土壌耐性 / トランスポーター / アルミニウム毒性 / マンガン毒性
Outline of Final Research Achievements

Toxicity of both aluminium (Al) and manganease (Mn) has been recognized as limiting factors of crop productivity on acidic soils. Buckwheat (Fagopyrum esculentum Moench) is a well-known acid soil tolerant species. Physiological study showed that buckwheat is also able to accumulate high Mn in the leave not only Al. The natural resistance-associated macrophage proteins (Nramps) constitute a large family in bacteria, fungi, plants and mammals. In this study report isolation and characterization of seven Nramp transporter genes in buckwheat. Heterologous experiment showed Mn concentration in yeast expressing FeNramp5 was higher than vector control and other lines. Futheremore expression of FeNramp5 in atnramp1, a mutant defective in Mn uptake in arabidopsis, rescued the phenotype. These results suggest that at least FeNramp5 is involved in the transport of Mn in buckwheat.

Academic Significance and Societal Importance of the Research Achievements

食糧不足問題を解決することは人類の抱える重要な課題の一つである。この問題の解決策のとして、これまでに農耕地として利用されていない、または生産性の低い問題土壌での作物の生産性の向上が期待されている。世界の耕地面積の3-4割は酸性土壌であり、そこでの主な生育阻害因子にアルミニウム毒性やマンガン毒性がある。
ソバは酸性土壌に強い植物であることが知られている。本研究ではソバが地上部にアルミニウムとマンガンを集積することを明らかにした。さらに、ソバのマンガン吸収に関与する輸送体を突き止めた。これはソバの酸性土壌耐性を理解するうえで重要な新しい知見であり、酸性土壌での作物の生産性向上に貢献する可能性がある。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Two Genes Encoding a Bacterial-Type ATP-Binding Cassette Transporter are Implicated in Aluminum Tolerance in Buckwheat2018

    • Author(s)
      Che Jing、Yamaji Naoki、Yokosho Kengo、Shen Ren Fang、Ma Jian Feng
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Pages: 2505-2511

    • DOI

      10.1093/pcp/pcy171

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Different roles of two variants of a half-size ABC transporter in Al accumulation and detoxification of buckwheat2018

    • Author(s)
      Lei, G. J., Yokosho, K., Yamaji, N. and Ma, J. F.
    • Organizer
      日本植物生理学会2018年度名古屋大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ソバのNrampファミリー遺伝子の機能解析2017

    • Author(s)
      横正健剛、邵継鋒、山地直樹、馬建鋒
    • Organizer
      日本土壌肥料学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Functional characterization of Nramp transporter genes in buckwheat.2017

    • Author(s)
      Yokosho, K., Shao, J. F., Yamaji, N., Shen, R. F. and Ma, J. F.
    • Organizer
      International Plant Nutrition Colloquium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2020-03-30  

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