| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Several cytoplasmic proteins such as elongation factor Tu (EF-Tu), which lack a typical N-terminal export signal sequence, have been found to be localized outside the cell in several Lactobacillus strains. EF-Tu often exert additional functions as a bacterial adhesion factor. However, the mechanism underlying the N-terminal typical signal sequence-independent export of these cytoplasmic proteins is largely unknown. Here we identified the secretion of EF-Tu depends on the C-terminal sequence containing certain hydrophobic amino acid residues and that the sequence contributes to the membrane export processes. This study improves our understanding of the Lactobacillus colonization strategy in the intestinal environment.
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