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Structural approach to the regulatory mechanism of strigolactone signaling

Research Project

Project/Area Number 17K15258
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Hirabayashi Kei  東京大学, 大学院農学生命科学研究科(農学部), 特別研究員 (00778730)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords植物ホルモン / X線結晶構造解析 / 構造生物学 / ストリゴラクトン
Outline of Final Research Achievements

Strigolactones (SLs) are plant hormones that regulate various physiological processes, such as shoot branching, root development and leaf senescence. Therefore, chemical regulation of SL functions is expected to be applied in agriculture.
In this study, we developed lead compounds of SL antagonists, which covalently bind to SL receptor D14, by preparing an array of triazole urea compounds. With subsequent biochemical and physiological analyses, we identified some derivatives which show stronger inhibitory activity. Intriguingly, we also found that one derivative shows agonistic activity promoting the interaction between D14 and its partner D53. These results indicate that triazole urea compounds are potentially powerful tools for agricultural application and are extremely useful for the elucidation of the complicated mechanism underlying SL signaling.

Academic Significance and Societal Importance of the Research Achievements

ストリゴラクトンは比較的最近になって発見された植物ホルモンで、まだまだ開発が遅れており、農業分野での応用研究が強く望まれている。しかしながら、自然界でのソースは限られており、合成コストも高いため、より単純で効率的な制御剤の開発が期待されている。
本研究で開発された新規制御剤は、簡便に合成できるだけでなく、阻害/促進の変換を可能とする画期的なものであり、農業適用にとって潜在的に強力なツールとなることは間違いない。またストリゴラクトン受容に関する複雑なメカニズムの解明(基礎研究の適用)にも極めて有用であることが予想される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (26 results)

All 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (15 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Remarks (2 results)

  • [Int'l Joint Research] King Abdullah University/of Science and Technology(サウジアラビア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] King Abdullah University/of Science and Technology(Saudi Arabia)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses2019

    • Author(s)
      Nakamura Hidemitsu、Hirabayashi Kei、Miyakawa Takuya、Kikuzato Ko、Hu Wenqian、Xu Yuqun、Jiang Kai、Takahashi Ikuo、Niiyama Ruri、Dohmae Naoshi、Tanokura Masaru、Asami Tadao
    • Journal Title

      Molecular Plant

      Volume: 12 Issue: 1 Pages: 44-58

    • DOI

      10.1016/j.molp.2018.10.006

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functions and Biosynthetic Systems of Iron-Sulfur Clusters2018

    • Author(s)
      HIRABAYASHI Kei
    • Journal Title

      Nihon Kessho Gakkaishi

      Volume: 60 Issue: 4 Pages: 165-166

    • DOI

      10.5940/jcrsj.60.165

    • NAID

      130007472782

    • ISSN
      0369-4585, 1884-5576
    • Year and Date
      2018-08-31
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rationally Designed Strigolactone Analogs as Antagonists of the D14 Receptor2018

    • Author(s)
      Takeuchi Jun、Jiang Kai、Hirabayashi Kei、Imamura Yusaku、Wu Yashan、Xu Yuqun、Miyakawa Takuya、Nakamura Hidemitsu、Tanokura Masaru、Asami Tadao
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Issue: 8 Pages: 1545-1554

    • DOI

      10.1093/pcp/pcy087

    • NAID

      120006549589

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for brassinosteroid response by BIL1/BZR12018

    • Author(s)
      Nosaki Shohei、Miyakawa Takuya、Xu Yuqun、Nakamura Akira、Hirabayashi Kei、Asami Tadao、Nakano Takeshi、Tanokura Masaru
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 10 Pages: 771-776

    • DOI

      10.1038/s41477-018-0255-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mapping the key residues of SufB and SufD essential for biosynthesis of iron-sulfur clusters2017

    • Author(s)
      Yuda Eiki、Tanaka Naoyuki、Fujishiro Takashi、Yokoyama Nao、Hirabayashi Kei、Fukuyama Keiichi、Wada Kei、Takahashi Yasuhiro
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 9387-9387

    • DOI

      10.1038/s41598-017-09846-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methyl phenlactonoates are efficient strigolactone analogs with simple structure2017

    • Author(s)
      Jamil M., Kountche A. B., Haider I., Guo X., Ntui O. V., Jia K., Ali S., Hameed S. U., Nakamura H., Lyu Y., Jiang K., Hirabayashi K., Tanokura M., Arold T. S., Asami T., Al-Babili S.
    • Journal Title

      Journal of Experimental Botany

      Volume: erx438 Issue: 9 Pages: 2319-2331

    • DOI

      10.1093/jxb/erx438

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of Quasi-Microgravity Environment for Protein Crystallization2017

    • Author(s)
      中村顕、平林佳、田之倉優
    • Journal Title

      Nihon Kessho Gakkaishi

      Volume: 59 Issue: 4 Pages: 182-187

    • DOI

      10.5940/jcrsj.59.182

    • NAID

      130006032034

    • ISSN
      0369-4585, 1884-5576
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Structural approach to the regulatory mechanism by novel covalent antagonists of strigolactone receptor2019

    • Author(s)
      Hirabayashi K., Nakamura H., Miyakawa T., Xu Y., Kikuzato K., Hu W., Jiang K., Asami T., Tanokura M.
    • Organizer
      The 1st International Symposium on the Chemical Communication (ISCC2019)(ポスター発表)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chemical regulation of strigolactone responses in plants2019

    • Author(s)
      Nakamura H., Hirabayashi K., Kikuzato K., Hu W., Niiyama R., Sakai T., Xu Y., Jiang K., Takahashi I., Dohmae N., Miyakawa T., Tanokura M., Asami T.
    • Organizer
      The 1st International Symposium on the Chemical Communication (ISCC2019)(ポスター発表)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ストリゴラクトン受容体アンタゴニストcarba-SLsの創出2018

    • Author(s)
      竹内純、姜凱、平林佳、今村優作、呉雅珊、徐玉群、宮川拓也、中村英光、田之倉優、浅見忠男
    • Organizer
      日本農薬学会第43回大会(口頭発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ストリゴラクトン情報伝達系における新規制御剤作用機構の構造基盤2018

    • Author(s)
      平林佳、宮川拓也、徐玉群、中村英光、喜久里貢、胡文倩、姜凱、浅見忠男、田之倉優
    • Organizer
      第91回日本生化学会年会(口頭発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物特異的なBZR転写因子ファミリーのDNA結合特異性の構造基盤2018

    • Author(s)
      野﨑 翔平、宮川拓也、徐玉群、中村顕、平林佳、浅見忠男、中野雄司、田之倉優
    • Organizer
      日本農芸化学会関東支部2018年度大会(口頭発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ブラシノステロイド情報伝達におけるマスター転写因子BIL1/BZR1のDNA結合特異性の構造基盤2018

    • Author(s)
      野﨑 翔平、宮川拓也、徐玉群、中村顕、平林佳、浅見忠男、中野雄司、田之倉優
    • Organizer
      植物化学調節学会第53回大会(口頭発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 生命活動に必須な金属補因子である鉄硫黄クラスターの組立における硫黄源の供給機構2018

    • Author(s)
      湯田瑛樹、平林佳、田中尚志、藤城貴史、横山奈央、福山恵一、湯井敏文、高橋康弘、和田啓
    • Organizer
      日本結晶学会平成30年度年会(口頭発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ストリゴラクトン情報伝達系における新規制御剤作用機構の構造基盤2018

    • Author(s)
      平林佳、宮川拓也、徐玉群、中村英光、喜久里貢、胡文倩、姜凱、浅見忠男、田之倉優
    • Organizer
      第91回日本生化学会年会(ポスター発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 超好熱性細菌の鉄硫黄クラスター生合成タンパク質群のX線結晶構造解析2018

    • Author(s)
      國近航平、藤城貴史、山川誠、平林佳、岩永朋子、福山恵一、和田啓、高橋康弘
    • Organizer
      第91回日本生化学会年会(ポスター発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物ホルモン・ストリゴラクトン情報伝達系を制御する新規薬剤の開発と作用機構2018

    • Author(s)
      平林佳、宮川拓也、徐玉群、中村英光、喜久里貢、胡文倩、姜凱、浅見忠男、田之倉優
    • Organizer
      日本結晶学会平成30年度年会(ポスター発表)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Novel Insights into Bacterial Iron-Sulfur Cluster Biosynthesis Systems2018

    • Author(s)
      Takahashi Y., Yokoyama N., Fujishiro T., Yuda E., Tanaka N., Hirabayashi K., Wada K.
    • Organizer
      Iron-Sulfur Proteins―Biogenesis, Regulation and Function (39th Steenbock Symposium)(口頭発表)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structural and Functional Analyses of E. coli SufBCD complex2018

    • Author(s)
      Wada K., Yuda E., Hirabayashi K., Tanaka N., Fujishiro T., Yokoyama N., Fukuyama K., Takahashi Y.
    • Organizer
      Iron-Sulfur Proteins―Biogenesis, Regulation and Function (39th Steenbock Symposium)(ポスター発表)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural basis for the regulation by novel chemical compounds involved in strigolactone signaling2018

    • Author(s)
      Hirabayashi K., Miyakawa T., Xu Y., Nakamura H., Kikuzato K., Hu W., Jiang K., Asami T., Tanokura M.
    • Organizer
      AsCA 2018/CRYSTAL 32(ポスター発表)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Crystal structure of the substrate bound form of biliverdin reductase with unpredicted substrate-stacked geometry revealed unique reaction mechanism2018

    • Author(s)
      Takao H., Hirabayashi K., Harada J., Sato H., Fukuyama K., Sugishima M., Wada K.
    • Organizer
      AsCA 2018/CRYSTAL 32(ポスター発表)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SufBCD複合体への変異導入解析から見えてきた鉄硫黄クラスターの生合成機構2017

    • Author(s)
      湯田瑛樹、田中尚志、藤城貴史、平林佳、福山恵一、和田啓、高橋康弘
    • Organizer
      ConBio2017(2017年度 生命科学系学会合同年次大会)
    • Related Report
      2017 Research-status Report
  • [Remarks] 植物の枝分かれをコントロールする新しい薬剤の開発

    • URL

      https://www.a.u-tokyo.ac.jp/topics/2018/20181122-1.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 植物ホルモンのマスター転写因子BIL1/BZR1の特異的な立体構造を解明

    • URL

      https://www.a.u-tokyo.ac.jp/topics/2018/20181002-1.html

    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2020-03-30  

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