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Engineered production and biosynthesis of mushroom-derived natural products from the encrypted biosynthetic gene

Research Project

Project/Area Number 17K15265
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bioorganic chemistry
Research InstitutionUniversity of Shizuoka

Principal Investigator

Tsunematsu Yuta  静岡県立大学, 薬学部, 講師 (30629697)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords生合成 / 担子菌 / 天然物 / 生合成遺伝子 / キノコ / 形質転換 / ポリケチド / テルペン / ウシグソヒトヨタケ / 二次代謝 / 強制発現 / 遺伝子破壊 / 休眠型遺伝子
Outline of Final Research Achievements

Mushrooms are used not only as food but also as traditional natural medicines (Kampo) and health supplements, and they have been shown to have various effects on the human body. In this study, we investigated the biosynthesis of mushroom-derived bioactive substances at the gene, enzyme, and compound levels, respectively. Increased production of bioactive substances could be accomplished by overexpression of mushroom genes. On the other hand, it was confirmed that the novel functional molecule obtained by the gene disruption method is a compound involved in the growth and differentiation of the mushroom. It may be a new tool that can be used for mushroom cultivation and breeding.

Academic Significance and Societal Importance of the Research Achievements

本研究ではキノコ(担子菌)への遺伝子導入や遺伝子破壊に挑戦した。これらの技術基盤を用いて生物活性物質の創製や生合成解明に向けた研究は国内外においてもほとんど認められない。それほどキノコの遺伝子操作は(特に化学者にとって)一般的で容易な技術ではないのである。異分野の研究協力者の助けを借り、失敗するリスクの高い研究内容に挑戦した。本研究にてキノコの形成・成長に関与する新規化合物Aが発見された点は特筆すべきである。今後、本新規化合物のキノコ形成に対する作用点の解明が進むことで効率的なキノコ栽培法の樹立が、さらにはマツタケなど現時点で栽培不能なキノコ種についても栽培化への道が拓かれることが期待される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (19 results)

All 2019 2018 2017

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Biosynthesis of lagopodins in mushroom involves a complex network of oxidation reactions2019

    • Author(s)
      Masuya Takahiro、Tsunematsu Yuta、Hirayama Yuichiro、Sato Michio、Noguchi Hiroshi、Nakazawa Takehito、Watanabe Kenji
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 17 Issue: 2 Pages: 234-239

    • DOI

      10.1039/c8ob02814a

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic Amide Tailoring Promotes Retro-Aldol Amino Acid Conversion To Form the Antifungal Agent Aspirochlorine2018

    • Author(s)
      Tsunematsu Yuta、Maeda Naoya、Yokoyama Mamoru、Chankhamjon Pranatchareeya、Watanabe Kenji、Scherlach Kirstin、Hertweck Christian
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 57 Issue: 43 Pages: 14051-14054

    • DOI

      10.1002/anie.201806740

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 新規疾患治療法開発のヒントは「微生物の生存戦略」に隠されている?2018

    • Author(s)
      恒松 雄太
    • Journal Title

      Farumashia

      Volume: 54 Issue: 5 Pages: 458-458

    • DOI

      10.14894/faruawpsj.54.5_458

    • NAID

      130006729840

    • ISSN
      0014-8601, 2189-7026
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of pex1 disruption on wood lignin biodegradation, fruiting development and the utilization of carbon sources in the white-rot Agaricomycete Pleurotus ostreatus and non-wood decaying Coprinopsis cinerea2017

    • Author(s)
      Nakazawa Takehito、Izuno Ayako、Horii Masato、Kodera Rina、Nishimura Hiroshi、Hirayama Yuichiro、Tsunematsu Yuta、Miyazaki Yasumasa、Awano Tatsuya、Muraguchi Hajime、Watanabe Kenji、Sakamoto Masahiro、Takabe Keiji、Watanabe Takashi、Isagi Yuji、Honda Yoichi
    • Journal Title

      Fungal Genetics and Biology

      Volume: 109 Pages: 7-15

    • DOI

      10.1016/j.fgb.2017.10.002

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Elucidation of biosynthetic pathways of natural products.2017

    • Author(s)
      Kishimoto, S., Tsunematsu, Y., Sato, M., Watanabe, K.
    • Journal Title

      The Chemical Record

      Volume: 印刷中 Issue: 11 Pages: 1095-1108

    • DOI

      10.1002/tcr.201700015

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 生合成遺伝子制御に基づく天然物創薬リバイバル2019

    • Author(s)
      恒松雄太
    • Organizer
      日本薬学会第139年会(千葉)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 複雑な酸化経路を含むキノコ由来lagopodin類の生合成研究2019

    • Author(s)
      桝谷貴洋, 平山裕一郎, 高西潤, 恒松雄太, 佐藤道大, 大高潤之介, 本山高幸, 長田裕之, 渡辺賢二
    • Organizer
      日本薬学会第139年会(千葉)
    • Related Report
      2018 Annual Research Report
  • [Presentation] レギュレータ-遺伝子改変法によるCoprinopsis属担子菌の新規天然物生合成経路活性化2019

    • Author(s)
      高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二
    • Organizer
      日本薬学会第139年会(千葉)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 複雑な酸化経路を含むキノコ由来lagopodin類の生合成研究2019

    • Author(s)
      桝谷貴洋, 平山裕一郎, 高西潤, 恒松雄太, 佐藤道大, 大高潤之介, 本山高幸, 長田裕之, 渡辺賢二
    • Organizer
      日本農芸化学会2019年度大会(東京)
    • Related Report
      2018 Annual Research Report
  • [Presentation] レギュレータ-遺伝子改変法によるCoprinopsis属担子菌の新規天然物生合成経路活性化2019

    • Author(s)
      高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二
    • Organizer
      日本農芸化学会2019年度大会(東京)
    • Related Report
      2018 Annual Research Report
  • [Presentation] キノコ由来天然物ラゴポジン類の生合成研究2018

    • Author(s)
      桝谷貴洋, 平山裕一郎, 高西潤, 田村優依, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二
    • Organizer
      日本生薬学会第65回年会(広島)
    • Related Report
      2018 Annual Research Report
  • [Presentation] キノコ由来二次代謝産物の過剰生産系の構築2018

    • Author(s)
      高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二
    • Organizer
      日本生薬学会第65回年会(広島)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Dissecting and harnessing fungal biosynthetic pathway2018

    • Author(s)
      恒松雄太, 渡辺賢二
    • Organizer
      International Congress on Pure & Applied Chemistry Langkawi (ICPAC Langkawi) 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Coprinopsis属担子菌のレギュレータ-遺伝子操作による化合物生産2018

    • Author(s)
      高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二
    • Organizer
      日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Dissecting and harnessing fungal biosynthetic pathway2018

    • Author(s)
      恒松雄太, 渡辺賢二
    • Organizer
      30th International Symposium on the Chemistry of Natural Products and the 10th International Congress on Biodiversity (ISCNP30 & ICOB10)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 糸状菌ゲノム情報を活用した新規天然物の探索とその生合成2018

    • Author(s)
      恒松雄太
    • Organizer
      静岡大学超領域研究推進本部 第12回超領域研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 冬虫夏草Cordyceps militarisにおけるcordycepin生合成経路の解析2017

    • Author(s)
      恒松雄太 天内優子 桝谷貴洋 松崎信生 崔宰熏 鈴木智大 道羅英夫 河岸洋和 渡辺賢二
    • Organizer
      日本生薬学会第64回年会(千葉)
    • Related Report
      2017 Research-status Report
  • [Presentation] キノコ由来生合成遺伝子の高度異種発現に基づく物質生産法の確立2017

    • Author(s)
      桝谷貴洋 平山裕一郎 松崎信生 恒松雄太 渡辺賢二
    • Organizer
      日本生薬学会第64回年会(千葉)
    • Related Report
      2017 Research-status Report
  • [Presentation] キノコ由来生合成遺伝子の高発現による物質生産2017

    • Author(s)
      桝谷貴洋 平山裕一郎 松崎信生 恒松雄太 佐藤道大 渡辺賢二
    • Organizer
      第17回糸状菌分子生物学コンファレンス
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-01-27  

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