Study of anti-obesity effect via eicosapentaenoic acid-induced improvement of skeletal muscle function
| Project/Area Number |
17K15272
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | EPA / エイコサペンタエン酸 / 筋細胞 / 骨格筋 / 魚油 / 肥満 / マイオカイン / PPARd / 脂肪酸 / PPARδ / 持久力 / 褐色脂肪 |
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| Outline of Final Research Achievements |
The purpose of this study was to elucidate the anti-obesity effect via eicosapentaenoic acid (EPA)-induced improvement of skeletal muscle function. First, to investigate the effects of EPA on muscle metabolism, L6 cells were treated with EPA and cell lysates were collected, and then metabolomic analysis was performed. The results showed that the metabolite profiles were different in control and EPA treated group. Next, to confirm the effects of metabolites released from muscle cells (myokines) on adipocytes, 3T3L1 cells were cultured in medium, collected from pre-cultured C2C12 myotubes with EPA. We found that EPA supplementation increased mRNA expression of lipid metabolism-related factors in 3T3L1 cells, however there was no effect on 3T3L1 cells culturing in medium obtained from myotubes with EPA.
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| Academic Significance and Societal Importance of the Research Achievements |
魚油の摂取は肝臓や脂肪組織に作用し、肥満改善に寄与することは知られているが、骨格筋を標的とした、臓器間ネットワークを介した研究は報告されていない。本研究は魚油に含まれるエイコサペンタエン酸（EPA）による骨格筋代謝能の向上に加え、骨格筋由来の代謝分泌物による脂肪細胞への影響、すなわち肥満改善をターゲットとした研究である。これは既存食品成分の新たな機能性を提唱するものである。また、運動トレーニングでなし得る身体への恩恵を食品成分で代替可能であることを示唆するものである。本研究の成果は筋機能への作用を謳った運動模倣サプリメントとして、肥満や運動不足が問題視されている現代社会への貢献が期待できる。
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Oleic acid up-regulates myosin heavy chain (MyHC) 1 expression and increases mitochondrial mass and maximum respiration in C2C12 myoblasts.2020
Author(s)
Watanabe, N., Komiya, Y., Sato, Y., Watanabe, Y., Suzuki, T., and Ariahara, K.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 525
Issue: 2
Pages: 406-411
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cell2017
Author(s)
R Tatsumi,T Suzuki, Mai-Khoi Q. Do, Y Ohya, JE. Anderson, A Shibata, M Kawaguchi, S Ohya, H Ohtsubo, W Mizunoya, S Sawano, Y Komiya, R Ichitsubo, K Ojima, S Nishimatsu, T Nohno, Y Ohsawa, Y Sunada, M Nakamura, M Furuse, Y Ikeuchi, T Nishimura, T Yagi, and R E. Allen
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Journal Title
STEM CELLS
Volume: 35
Issue: 7
Pages: 1815-1834
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Fast-to-slow shift of muscle fiber-type composition by dietary apple polyphenols in rats: Impact of the low-dose supplementation2017
Author(s)
Mizunoya W, Okamoto S, Miyahara H, Akahoshi M, Suzuki T, Do MKQ, Ohtsubo H, Komiya Y, Qahar M, Waga T, Nakazato K, Ikeuchi Y, Anderson JE, Tatsumi R
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Journal Title
Animal Science Journal
Volume: 88
Issue: 3
Pages: 489-499
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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