Improvement of in vitro embryo production by autophagy regulation in cattle
| Project/Area Number |
17K15361
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 体外受精 / 胚発生 / オートファジー / ウシ / 小胞体ストレス / リソソーム / 畜産学 |
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| Outline of Final Research Achievements |
To improve the developmental competence of bovine embryos produced in vitro, this study focused on autophagy occurring in bovine oocytes and embryos, and investigated the relationship between autophagy dynamics and developmental competence in early embryogenesis. As a result of observing the dynamics of autophagy activity, the activity was increased during early embryonic stages. Moreover, our data indicated that the dynamics were different from those in mice, suggesting that it would differ between species. Next, we conducted experiments to inhibit or induce autophagy in a time-specific manner. Induction of autophagy during early embryonic stages improved the developmental rate to blastocyst stage, whereas this rate drastically decreased by inhibition of autophagy. In conclusion, this study indicated that autophagy regulation during in vitro development could improve the developmental competence of in vitro produced embryos.
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| Academic Significance and Societal Importance of the Research Achievements |
胚発生過程といった特殊な時期におけるオートファジー機構には不明な点が数多く残されており、本研究の成果は学術的意義が高いといえる。また、近年の人工授精による受胎率の低下により、体外生産胚の胚移植の実施件数が世界的に急増しており、それに伴う体外生産胚の高品質化に対する畜産業界からの期待は非常に高い。すなわち、オートファジー動態の詳細を明らかにし、それらの制御を通じて体外生産胚の高品質化を示した本研究の成果は、胚移植による子牛生産の基盤技術として期待されている体外胚生産系の高度化に重要な知見であり、学術的のみならず、実社会での課題解決に貢献できるという点で社会的意義が高いといえる。
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility2018
Author(s)
Maria Portia B. Nagata, Kenji Endo, Kazuko Ogata, Kenichi Yamanaka, Junki Egashira, Naoto Katafuchi, Tadayuki Yamanouchi, Hideo Matsuda, Yuki Goto, Miki Sakatani, Takuo Hojo, Hirofumi Nishizono, Kenji Yotsushima, Naoki Takenouchi, Yutaka Hashiyada, Kenichi Yamashita
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 115
Issue: 14
Pages: 3087-3096
DOI
Related Report
Peer Reviewed / Open Access
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