| Project/Area Number |
17K15362
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Ishida Aiko 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 上級研究員 (30414684)
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| Project Period (FY) |
2017-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Cat-2 / アルギニン / ブタ / 骨格筋 / 塩基性アミノ酸トランスポーター2 / 筋線維型 / NO / CAT-2 / NOS |
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| Outline of Final Research Achievements |
Dietary arginine concentration changed expression level of myosin heavy chain isoform in skeletal muscles of pigs without changes of expression of cationic amino acid transporter (Cat)-2 mRNA. It suggests that changes in Cat-2 mRNA expression are not essential for changes in muscle fiber type. The effect on myosin heavy chain (MyHC) isoform are different between the experiment in pigs with dietary arginine level and the experiment in cultured C2C12 cells with arginine concentration of medium, which is suggests that the effect of in pigs are mediated by endocrine. In C2C12 cells, arginine concentration of medium and clenbuterol affects the expression of Cat-2A and Cat-2B mRNA expression during differentiation, but not after differentiation. Clenbuterol promoted the expression of Cat-2 mRNA, which suggests that β2 -adrenergic receptor mediated stimulation is involved in the regulation of Cat-2 mRNA expression.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで、Cat-2はマクロファージや腫瘍細胞におけるNO産生時に、基質となるアルギニンを供給するトランスポーターとして研究が進められてきた。Cat-2の骨格筋における研究はこれまでに報告が少なく、発現量の変化や制御については明らかになっていなかった。本研究は栄養により骨格筋のCat-2発現量に及ぼす影響を明らかにした研究である。さらに、培養骨格筋細胞を用いて、アルギニンの直接的な影響、また発現制御まで検討したことは、学術的意義がある。
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