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Understanding the human infectivity in Tyrpanosoma species

Research Project

Project/Area Number 17K15366
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionHokkaido University

Principal Investigator

Hayashida Kyoko  北海道大学, 人獣共通感染症リサーチセンター, 特任助教 (40615514)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsトリパノソーマ原虫 / 進化 / ゲノム / 人獣共通感染症 / アフリカトリパノソーマ / Trypanosoma evansi / スーラ / ズーノーシス / 分子進化 / トリパノソーマ / 血清抵抗性 / トランスクリプトーム / 野外分離株
Outline of Final Research Achievements

Human had evolved to acquire the natural immunity against Trypanosoma infection, called Trypanolytic factor (TLF) in its serum. On the other hand, two Trypanosome species evolved to acquire the mechanism to combat with human anti-Trypanosome immune system, allowing to infect humans. To better understand of host-parasite interaction of the Trypanozoon parasite, we adapted T. evansi parasite into human-serum added media in vitro. Genome-wide transcription analysis using RNA-seq revealed that, several genes are differentially expressed comparing the original strain in these human-serum resistant parasite clones. Notably, the expression of HpHbR gene, that encodes the parasite receptor of TLF was significantly downregulated. This result provided a possible mechanism of African Trypanosoma to acquire human serum resistance, and proposed the potential risk of animal-infective Trypanosoma to become an potential zoonotic parasite.

Academic Significance and Societal Importance of the Research Achievements

ヒトのアフリカ睡眠病を引き起こすTrypanosoma brucei と、家畜の病原体であるT. evansiは近縁関係にあるが後者はヒト血清抵抗性を有さない。本研究ではT. evansiを試験管内でヒト血清へ馴化し網羅的遺伝子解析を行った。結果、ヒトの抗トリパノソーマ因子TLFのレセプターHpHbRの発現が血清抵抗性T. evansiで減少していることを明らかにした。本知見はヒト血清抵抗性の獲得という現象が、Trypanozoon亜属に共通して起こりうる現象であることを示唆する。T. evansiが新たな人獣共通感染症の原因となりうるのか、今後慎重に検討すべき課題であると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2020 2019

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (1 results)

  • [Journal Article] First molecular identification of Trypanosoma evansi from cattle in Syria2020

    • Author(s)
      Marsela M, Hayashida K, Terkawi A, Xuan X, Sugimoto C, Yamagishi J
    • Journal Title

      Japanese Journal of Veterinary Research

      Volume: 68(2) Pages: 117-128

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies.2020

    • Author(s)
      Gaithuma A, Yamagishi J, Hayashida K, Kawai N, Namangala B, Sugimoto C.
    • Journal Title

      Scientific Reports

      Volume: 10(1) Issue: 1 Pages: 5005-5005

    • DOI

      10.1038/s41598-020-61817-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic diversity and population structure of Glossina morsitans morsitans in the active foci of human African trypanosomiasis in Zambia and Malawi2019

    • Author(s)
      Yukiko Nakamura, Junya Yamagishi, Kyoko Hayashida, Naoki Osada, Elisha Chatanga, Cornelius Mweempwa, Kalinga Chilongo, John Chisi, Jenelisa Musaya, Ryosuke Omori, Noboru Inoue, Boniface Namangala, Chihiro Sugimoto
    • Journal Title

      PLoS Negl. Trop. Dis

      Volume: 13 Issue: 7 Pages: e0007568-e0007568

    • DOI

      10.1371/journal.pntd.0007568

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A single test approach for accurate and sensitive detection and taxonomic characterization of Trypanosomes by comprehensive analysis of internal transcribed spacer 1 amplicons.2019

    • Author(s)
      Gaithuma AK, Yamagishi J, Martinelli A, Hayashida K, Kawai N, Marsela M, Sugimoto C.
    • Journal Title

      PLoS Neglected Tropical Diseases.

      Volume: 13(2) Issue: 2 Pages: e0006842-e0006842

    • DOI

      10.1371/journal.pntd.0006842

    • NAID

      120006626577

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Trypanosoma evansi におけるヒト血清抵抗性獲得の分子機構解明2019

    • Author(s)
      林田京子、山岸潤也、杉本千尋
    • Organizer
      第88回日本寄生虫学会
    • Related Report
      2019 Annual Research Report 2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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