| Project/Area Number |
17K15384
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
Miura Ryotaro 日本獣医生命科学大学, 獣医学部, 講師 (60782133)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 牛 / 第1卵胞波主席卵胞 / 黄体 / 子宮内膜 / 遺伝子発現 / 代謝 / ウシ / 子宮 / 卵巣 / 子宮機能 / 胚発育 / 受胎性 / 獣医学 |
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| Outline of Final Research Achievements |
In the present study, to clarify the causes of lower fertility in cows with ipsilateral relationship of the first wave dominant follicle and corpus luteum in the same ovary, we analyzed the amounts of endometrium mRNA by real-time PCR and postpartum metabolic status by milk yield and milk components between ipsilateral group (IG) and contralateral group (CG). Amounts of PGR、PGRMC1、ESR1、PGFS and PPARA mRNA were higher in CG than IG. Negative energy balance of postparum status was more severe in IG than CG. From these results, it is possible that the cows with more severe negative energy balance lead the ipsilateral relationship of the first wave dominant follicle and corpus luteum in the same ovary. Also, it is speculated that the difference of amounts of mRNA in endometrium were caused by difference of postpartum metebolic status.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は発情周期中の第1卵胞波主席卵胞および黄体の同一卵巣内での共存が人工授精後の受胎率低下をもたらすメカニズムを明らかにすることであるが、結果から共存と非共存では子宮内膜機能に差異がある可能性が示されたが、一方で分娩後の代謝状態が異なっていることも示唆された。これは第1卵胞波主席卵胞と黄体の同一卵巣内での共存が受胎性を低下させるのではなく、共存をもたらす牛の代謝状態が受胎性低下をもたらしてることが推測され、牛の卵巣状態を観察することで繁殖性向上につながる飼養管理を評価出来る可能性があり、生産現場における牛の受胎性の向上につながる応用研究および技術開発をもたらすと考えている。
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