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Pathogenicity analysis of bovine leukemia virus

Research Project

Project/Area Number 17K15385
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionAzabu University

Principal Investigator

Murakami Hironobu  麻布大学, 獣医学部, 助教 (60620929)

Research Collaborator Sato Reiichiro  
Uchiyama Jumpei  
Maeda Yosuke  
Kato Hajime  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords牛白血病ウイルス / 地方病性牛白血病 / 分子クローン / 病原性 / 伝播性 / 遺伝的多様性 / 感染性分子クローン / ウイルス産生量 / 形質転換 / 野外株 / 牛白血病 / 遺伝的多型
Outline of Final Research Achievements

To research whether a genetic variation affects viral properties, phylogenetic analysis was performed using whole BLV genome and revealed to be classified three group in BLV wild-type strains. In addition, the groups were associated with virus productivity. The one of three group strains classified in this study showed high virus productivity and had an advantage for transmissibility in field. Moreover, pathogenesis was different among wild-type strains, but some strains harbored deletion mutation and showing low viral production had high pathogenesis. Taken together, transmissibility and pathogenesis would be independently decided by different genetic factors.

Academic Significance and Societal Importance of the Research Achievements

本研究により、BLVの遺伝的多様性がウイルス性状を変化させ、異なるウイルス産生能力や形質転換能力を示させることが明らかとなった。さらに、これらウイルス性状の変化は伝播性や病原性と関連することが示唆された。これらのことから、特定の株で高伝播性や高病原性となるため、株により経済的損失リスクが異なることが考えられた。したがって、本研究成果は、BLV感染拡大およびEBL発症リスクを評価することによる新たなBLV制御対策へ応用可能であると考えられる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (19 results)

All 2019 2018 2017

All Journal Article (10 results) (of which Peer Reviewed: 9 results,  Open Access: 7 results) Presentation (9 results) (of which Invited: 3 results)

  • [Journal Article] Variations in the viral genome and biological properties of bovine leukemia virus wild-type strains2018

    • Author(s)
      Murakami Hironobu、Uchiyama Jumpei、Suzuki Chihiro、Nikaido Sae、Shibuya Kaho、Sato Reiichiro、Maeda Yosuke、Tomioka Michiko、Takeshima Shin-nosuke、Kato Hajime、Sakaguchi Masahiro、Sentsui Hiroshi、Aida Yoko、Tsukamoto Kenji
    • Journal Title

      Virus Research

      Volume: 253 Pages: 103-111

    • DOI

      10.1016/j.virusres.2018.06.005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新たなアプローチによる地方病性牛白血病の病態解釈2018

    • Author(s)
      村上裕信
    • Journal Title

      臨床獣医

      Volume: 36 Pages: 38-43

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Analysis of transmissibility and pathogenesis in bovine leukemia virus2018

    • Author(s)
      村上裕信
    • Journal Title

      Electrophoresis Letters

      Volume: 62 Issue: 2 Pages: 49-54

    • DOI

      10.2198/electroph.62.49

    • NAID

      130007546948

    • ISSN
      2189-2628, 2189-2636
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Virus purification by CsCl density gradient using general centrifugation.2018

    • Author(s)
      Nasukawa T, Uchiyama J, Taharaguchi S, Ota S, Ujihara T, Matsuzaki S, Murakami H, Mizukami K, Sakaguchi M.
    • Journal Title

      Archives of Virology

      Volume: 162 Issue: 11 Pages: 3523-3528

    • DOI

      10.1007/s00705-017-3513-z

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a luminescence syncytium induction assay (LuSIA) for easily detecting and quantitatively measuring bovine leukemia virus infection.2018

    • Author(s)
      Sato H, Watanuki S, Murakami H, Sato R, Ishizaki H, Aida Y.
    • Journal Title

      Archives of Virology

      Volume: in press Issue: 6 Pages: 1519-1530

    • DOI

      10.1007/s00705-018-3744-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan.2018

    • Author(s)
      Ujihara T, Uchiyama J, Nasukawa T, Ando H, Murakami H, Ohara N, Ogawa M, Yamazaki T, Daibata M, Sakaguchi M, Matsuzaki S.
    • Journal Title

      Archives of Virology

      Volume: in press Issue: 7 Pages: 1-5

    • DOI

      10.1007/s00705-018-3788-8

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Piperacillin and ceftazidime produce the strongest synergistic phage-antibiotic effect in Pseudomonas aeruginosa.2018

    • Author(s)
      Uchiyama J, Shigehisa R, Nasukawa T, Mizukami K, Takemura-Uchiyama I, Ujihara T, Murakami H, Imanishi I, Nishifuji K, Sakaguchi M, Matsuzaki S.
    • Journal Title

      Archives of Virology

      Volume: in press Issue: 7 Pages: 1-8

    • DOI

      10.1007/s00705-018-3811-0

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A nasal osteoma with an acute course in a Japanese Black heifer2017

    • Author(s)
      R. Sato, Y. Une, H. Madarame, H. Hanami, E. Kanai, H. Murakami, A. Tsukamoto, T. Suzuki, H. Ochiai, M. Kikuchi, H. Tanaka, K. Onda.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 7 Pages: 1220-1224

    • DOI

      10.1292/jvms.17-0041

    • NAID

      130005808392

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bovine leukemia virus G4 enhances virus production.2017

    • Author(s)
      Murakami H, Asano S, Uchiyama J, Sato R, Sakaguchi M, Tsukamoto K.
    • Journal Title

      Virus Research

      Volume: 238 Pages: 213-217

    • DOI

      10.1016/j.virusres.2017.07.005

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adsorption of Staphylococcus viruses S13´ and S24-1 on Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids.2017

    • Author(s)
      Uchiyama, J., Taniguchi, M., Kurokawa, K., Takemura-Uchiyama, I., Ujihara, T., Shimakura, H., Sakaguchi, Y., Murakami, H., Sakaguchi, M., Matsuzaki, S.
    • Journal Title

      J. Gen. Virol.

      Volume: 98(8) Issue: 8 Pages: 2171-2180

    • DOI

      10.1099/jgv.0.000865

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 牛白血病ウイルス野生株の特徴とBLV対策への活用2019

    • Author(s)
      村上裕信
    • Organizer
      平成30年度 日本獣医師会獣医学術学会年次大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 新たなアプローチによる地方病性牛白血病の病態解釈2018

    • Author(s)
      村上裕信
    • Organizer
      第124 回日本畜産学会関連集会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 牛白血病ウイルスの伝播性・病原性解析2018

    • Author(s)
      村上裕信
    • Organizer
      第68回日本電気泳動学会シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 牛白血病ウイルス野外株の遺伝的多様性と病原性への影響2018

    • Author(s)
      村上裕信、河合将克、佐藤礼一郎、前田洋佑、加藤肇、内山淳平、阪口雅弘、塚本健司
    • Organizer
      第161回日本獣医学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 牛白血病ウイルス野外株のウイルス産生量を増強する領域の探索2018

    • Author(s)
      戸髙 晴菜、鈴木千裕、二階堂紗恵、佐藤礼一郎、塚本健司、村上裕信
    • Organizer
      第2回日本ワンヘルスサイエンス学会年次学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 村上舞琴、紙透伸治、塚本健司、村上裕信2017

    • Author(s)
      牛白血病ウイルスの感染阻害に関与する化合物のスクリーニング
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 牛白血病ウイルス野外株の病原性解析2017

    • Author(s)
      河合将克、加藤 肇、前田洋祐、長谷部文子、泉對 博、二階堂紗恵、鈴木千裕、渋谷夏帆、佐藤礼一郎、武田 章、 佐藤一彦、山崎政司、高橋博文、田畑裕二、塚本健司、村上裕信
    • Organizer
      獣医学術学会年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 牛白血病ウイルス感染阻害剤の探索2017

    • Author(s)
      村上舞琴、紙透伸治、長谷部文子、田畑裕二、塚本健司、 村上裕信
    • Organizer
      獣医学術学会年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 遺伝的変化がもたらす牛白血病ウイルスの伝播力への影響2017

    • Author(s)
      村上裕信、内山淳平、鈴木千裕、二階堂紗恵、渋谷夏帆、 佐藤礼一郎、前田洋佑、富岡美千子、加藤肇、竹嶋伸之輔、泉對博、間陽子、塚本健司
    • Organizer
      獣医学術学会年次大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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