| Project/Area Number |
17K15387
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Fukunaga Koya 国立研究開発法人理化学研究所, 生命医科学研究センター, 研究員 (50506722)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 乳房炎 / 牛 / MHC / GWAS / BoLA / 黄色ブドウ球菌 / ゲノムワイド関連解析 / 黄色ブドウ球菌性乳房炎 / ホルスタイン / 慢性感染 / 乳房炎の慢性化 / 全ゲノム関連解析 |
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| Outline of Final Research Achievements |
The objective was to identify the susceptibility of staphylococcus aureus-induced mastitis in Holstein cattle and to estimate the risk using genome wide association study (GWAS). As a result, the association of the loci on the BTA7 and BTA26 with chronicity of staphylococcus aureus-induced mastitis was found. In BTA26 region, ACSL5, which was the long-chain fatty-acid-coenzyme A, might associate with the chronicity. Using GWAS, we could not identify the alleles of six genes in major histocompatibility complex (MHC), class II. Therefore, the genotyping method to identify all alleles of six genes containing copy number variation using the next generation sequencer were developed. Using this method, the weak association of BoLA-DRB3*011:01 with the susceptibility of staphylococcus aureus-induced mastitis were clarified. In the future, this method to genotype the BoLA alleles could be a gold standard to identify the genomic biomarker associated with the other contagious diseases.
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| Academic Significance and Societal Importance of the Research Achievements |
同定したゲノムバイオマーカーを用いたゲノム選抜を行うことで牛の黄色ブドウ球菌性乳房炎に耐性のもつ牛を選定することが可能となり、乳製品の生産性の向上が期待される。さらに同定された遺伝子は既存の乳房炎のメカニズムにはない新規知見であり、乳房炎の治療撲滅の一助となるだろう。また、GWASでは調べることのできないBoLA領域の6遺伝子の網羅的なジェノタイピング法を構築したことは今後、他の感染症のゲノムバイオマーカーの同定のための貴重なツールである。
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