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Genome wide association study to identify the susceptibility of staphylococcus aureus-induced mastitis in Holstein cattle and to estimate the risk

Research Project

Project/Area Number 17K15387
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Fukunaga Koya  国立研究開発法人理化学研究所, 生命医科学研究センター, 研究員 (50506722)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords乳房炎 / 牛 / MHC / GWAS / BoLA / 黄色ブドウ球菌 / ゲノムワイド関連解析 / 黄色ブドウ球菌性乳房炎 / ホルスタイン / 慢性感染 / 乳房炎の慢性化 / 全ゲノム関連解析
Outline of Final Research Achievements

The objective was to identify the susceptibility of staphylococcus aureus-induced mastitis in Holstein cattle and to estimate the risk using genome wide association study (GWAS). As a result, the association of the loci on the BTA7 and BTA26 with chronicity of staphylococcus aureus-induced mastitis was found. In BTA26 region, ACSL5, which was the long-chain fatty-acid-coenzyme A, might associate with the chronicity. Using GWAS, we could not identify the alleles of six genes in major histocompatibility complex (MHC), class II. Therefore, the genotyping method to identify all alleles of six genes containing copy number variation using the next generation sequencer were developed. Using this method, the weak association of BoLA-DRB3*011:01 with the susceptibility of staphylococcus aureus-induced mastitis were clarified. In the future, this method to genotype the BoLA alleles could be a gold standard to identify the genomic biomarker associated with the other contagious diseases.

Academic Significance and Societal Importance of the Research Achievements

同定したゲノムバイオマーカーを用いたゲノム選抜を行うことで牛の黄色ブドウ球菌性乳房炎に耐性のもつ牛を選定することが可能となり、乳製品の生産性の向上が期待される。さらに同定された遺伝子は既存の乳房炎のメカニズムにはない新規知見であり、乳房炎の治療撲滅の一助となるだろう。また、GWASでは調べることのできないBoLA領域の6遺伝子の網羅的なジェノタイピング法を構築したことは今後、他の感染症のゲノムバイオマーカーの同定のための貴重なツールである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] BoLA-DRB3 polymorphism is associated with differential susceptibility to bovine leukemia virus-induced lymphoma and proviral load.2020

    • Author(s)
      Lo C-W, Borjigin L, Saito S, Fukunaga K., Saitou E, Okazaki K, Mizutani T, Wada S, Takeshima S, Aida Y
    • Journal Title

      Viruses

      Volume: 12 Issue: 3 Pages: 352-352

    • DOI

      10.3390/v12030352

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genomewide Association Study Confirming the Association of NAT2 with Susceptibility to Antituberculosis Drug-Induced Liver Injury in Thai Patients2019

    • Author(s)
      Suvichapanich S, Wattanapokayakit S, Mushiroda T, Yanai H, Chuchottawon C, Kantima T, Nedsuwan S, Suwankesawong W, Sonsupap C, Pannarunothai R, Tumpattanakul S, Bamrungram W, Chaiwong A, Mahasirimongkol S, Mameechai S, Panthong W, Klungtes N, Munsoo A, Chauychana U, Maneerat M, Fukunaga K, Omae Y, Tokunaga K
    • Journal Title

      Antimicrobial Agents and Chemotherapy

      Volume: 63 Issue: 8 Pages: 2692-2718

    • DOI

      10.1128/aac.02692-18

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] GSTP1 rs1695 is associated with both hematological toxicity and prognosis of ovarian cancer treated with paclitaxel plus carboplatin combination chemotherapy: a comprehensive analysis using targeted resequencing of 100 pharmacogenes2018

    • Author(s)
      Yoshihama T, Fukunaga K, Hirasawa A, Nomura H, Akahane T, Kataoka F, Yamagami W, Aoki D, Mushiroda T
    • Journal Title

      Oncotarget

      Volume: 9 Issue: 51 Pages: 29789-29800

    • DOI

      10.18632/oncotarget.25712

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ウシMHCクラスⅡ 6遺伝子のコピー数とNGS解析を組み合わせた新規アリル同定およびそのハプロタイプ構造の推定2020

    • Author(s)
      福永航也、山下祐輔、八木沢拓也
    • Organizer
      第29回日本組織適合性学会
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 薬物動態関連遺伝子の網羅的配列解析法とそれに使用 されるプライマーセット2019

    • Inventor(s)
      莚田泰誠、福永航也
    • Industrial Property Rights Holder
      莚田泰誠、福永航也
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-001821
    • Filing Date
      2019
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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