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Development of the evaluation method and elucidation of molecular basis of zygotic totipotency

Research Project

Project/Area Number 17K15394
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Integrative animal science
Research InstitutionUniversity of Yamanashi

Principal Investigator

OOGA Masatoshi  山梨大学, 大学院総合研究部, 助教 (40644886)

Research Collaborator WAKAYAMA Teruhiko  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords個体形成能
Outline of Final Research Achievements

Zygotes which are derived from sperm and oocytes, can give rise to offspring. However, not all of them can be children: there are two types of zygotes, one can develop to full-term but another one can not. Here, I am trying to establish the technique which can distinguish the former from the later. Importantly the methods needs to be able to distinguish them without killing.

Academic Significance and Societal Importance of the Research Achievements

一見同じように見える1細胞期胚を、将来子になるものとなれないものとに生きたまま区別することができることには以下のような意義がある。学術的には、生まれられる胚と途中で死んでしまう胚の比較解析が可能となり、遺伝子発現レベルではどのような違いがあるのかという疑問の解明に繋がる。また、社会的には、母体に移植する前の胚の中から最良の胚を選択できるようになれば、不妊治療ならびに畜産部門においてこの知見は非常に価値あるものとなると考えている。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Zygotic fluorescence recovery after photo-bleaching analysis for chromatin looseness that allows full-term development2018

    • Author(s)
      Ooga Masatoshi、Funaya Satoshi、Aoki Fugaku、Wakayama Teruhiko
    • Journal Title

      Journal of visualized experiments

      Volume: ー Issue: 136 Pages: 136-136

    • DOI

      10.3791/57068

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chd9 mediates highly loosened chromatin structure in growing mouse oocytes2018

    • Author(s)
      Ooga Masatoshi、Funaya Satoshi、Hashioka Yuki、Fujii Wataru、Naito Kunihiko、Suzuki Masataka G.、Aoki Fugaku
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 500 Issue: 3 Pages: 583-588

    • DOI

      10.1016/j.bbrc.2018.04.105

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] FRAP analysis of chromatin looseness in mouse zygotes that allows full-term development2017

    • Author(s)
      Ooga Masatoshi、Wakayama Teruhiko
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 5 Pages: e0178255-e0178255

    • DOI

      10.1371/journal.pone.0178255

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ROSI胚におけるepigenetic parental asymmetryの消失と回復2018

    • Author(s)
      大我政敏、風間晃輔、上村悟氏、若山照彦
    • Organizer
      繁殖生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Establishment of zygotic fluorescence recovery after photobleaching for ROSI-derived zygotes2018

    • Author(s)
      Masatoshi Ooga, Kousuke Kazama and Teruhiko Wakayama
    • Organizer
      The international Conference on Cell Reprogramming and Reproduction
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マウス円形精子細胞注入胚の低産仔率の原因究明と向上の試み2018

    • Author(s)
      風間晃輔、大我政敏、上村悟氏、若山照彦
    • Organizer
      日本卵子学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Abnormal chromatin structure in the male pronucleus fertilized with Round spermatid2018

    • Author(s)
      Masatoshi Ooga、Teruhiko Wakayama
    • Organizer
      Epigenomics Data Analysis Core Presents Seminar
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] マウス円形精子細胞注入胚の低産仔率の原因究明と向上の試み2018

    • Author(s)
      風間晃輔、大我政敏、上村悟氏、若山照彦
    • Organizer
      日本哺乳類卵子学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Disrupted parental asymmetry of chromatin structure in ROSI-derived zygotes2017

    • Author(s)
      Masatoshi Ooga、Kousuke Kazama、 Satoshi Kamimura、Teruhiko Wakayama
    • Organizer
      World congress of reproductive biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2020-03-30  

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