Physiological and molecular analysis of cysteine hydropersulfide produced by cysteinyl-tRNA synthetase in yeast
| Project/Area Number |
17K15408
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
Takagi Hiroshi
|
|---|
| Project Period (FY) |
2017-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
|---|
| Keywords | 活性イオウ分子 / システインパーサルファイド / 寿命 / システニルRNA合成酵素 / 酵母 / 出芽酵母 / システインパースルフィド / システニルtRNA合成酵素 / 活性イオウ種 / ミトコンドリア |
|---|
| Outline of Final Research Achievements |
We attempted to understand physiological functions of reactive sulfur species (RSS) using yeast Saccharomyces cerevisiae, a useful model for eukaryotic biology. First, we found that yeast has a pyridoxal phosphate (PLP)-dependent biosynthesis of RSS by cysteinyl-tRNA synthetase (CARS), similar to bacteria and mammal. Next, our study indicated that a yeast mutant of PLP-binding site, which possessed the intact protein synthesis, dramatically reduces chronological aging, compared with wild-type, suggesting that CARS-produced RSS may play an important role in regulating longevity and sustaining the life span.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、ヒトの抗老化戦略の構築のみならず、エネルギー代謝異常が関わる各種疾患（ミトコンドリア病、生活習慣病、がん等）の発症機構の理解と予防・治療法の開発に大きく寄与することが期待される。また、活性イオウ分子の産生系をターゲットにした抗真菌薬の開発も抗菌・真菌薬開発も期待される。
|
Report
(3 results)
Research Products
(32 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] レドックス疾患学2018
Author(s)
井田 智章、西村 明、守田 匡伸
Total Pages
276
Publisher
羊土社
ISBN
9784758103695
Related Report
-
-
-
