Physiological and molecular analysis of cysteine hydropersulfide produced by cysteinyl-tRNA synthetase in yeast
| Project/Area Number |
17K15408
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Research Collaborator |
Takagi Hiroshi
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 活性イオウ分子 / システインパーサルファイド / 寿命 / システニルRNA合成酵素 / 酵母 / 出芽酵母 / システインパースルフィド / システニルtRNA合成酵素 / 活性イオウ種 / ミトコンドリア |
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| Outline of Final Research Achievements |
We attempted to understand physiological functions of reactive sulfur species (RSS) using yeast Saccharomyces cerevisiae, a useful model for eukaryotic biology. First, we found that yeast has a pyridoxal phosphate (PLP)-dependent biosynthesis of RSS by cysteinyl-tRNA synthetase (CARS), similar to bacteria and mammal. Next, our study indicated that a yeast mutant of PLP-binding site, which possessed the intact protein synthesis, dramatically reduces chronological aging, compared with wild-type, suggesting that CARS-produced RSS may play an important role in regulating longevity and sustaining the life span.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、ヒトの抗老化戦略の構築のみならず、エネルギー代謝異常が関わる各種疾患（ミトコンドリア病、生活習慣病、がん等）の発症機構の理解と予防・治療法の開発に大きく寄与することが期待される。また、活性イオウ分子の産生系をターゲットにした抗真菌薬の開発も抗菌・真菌薬開発も期待される。
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Polysulfide stabilization by tyrosine and hydroxyphenyl-containing derivatives that is important for a reactive sulfur metabolomics analysis2019
Author(s)
Hamid HA, Tanaka A, Ida T, Nishimura A, Matsunaga T, Fujii S, Morita M, Sawa T, Fukuto JM, Nagy P, Tsutsumi R, Motohashi H, Ihara H, Akaike T.
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Journal Title
Redox Biol.
Volume: 21
Pages: 101096-101096
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Book] レドックス疾患学2018
Author(s)
井田 智章、西村 明、守田 匡伸
Total Pages
276
Publisher
羊土社
ISBN
9784758103695
Related Report
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