Elucidation of plant innate immunity regulated by plasma membrane microdomains

• Project/Area Number: 17K15412
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Ritsumeikan University (2018-2020); Saitama University (2017)
• Principal Investigator: Nagano Minoru 立命館大学, 生命科学部, 助教 (80598251)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 植物免疫 / 細胞膜 / マイクロドメイン / スフィンゴ脂質 / 植物 / 免疫

Outline of Final Research Achievements

To reveal the role of plasma membrane microdomains in plant innate immunity, we established the Arabidopsis transgenic lines and mutants in which microdomains were decreased. Proteome analysis showed that major immune proteins existed on microdomains. We also demonstrated that microdomains contributed to the generation of reactive oxygen species during disease response. These results suggest that microdomains play an important role in Arabidopsis immunity.

Academic Significance and Societal Importance of the Research Achievements

本研究により、恒常的にマイクロドメインが減少したシロイヌナズナ系統を作出することができたため、マイクロドメインに関する生化学実験や生理学実験を容易に行うことが可能となった。そのため、マイクロドメインの植物免疫における役割を解明することができた。マイクロドメインは免疫以外にも様々な環境ストレス応答や生育に関与することが示唆されているため、本研究で確立した実験系は他の生理学的機能とマイクロドメインの関係の解明にも展開できる。またマイクロドメインの機能が解明できれば、マイクロドメインを利用した植物機能の強化が期待できる。

Research Products

• [Int'l Joint Research] ボルドー大学/フランス国立科学研究センター(フランス)
• [Int'l Joint Research] ボルドー大学/フランス国立科学研究センター(フランス)
• [Int'l Joint Research] ボルドー大学/フランス国立科学研究センター(フランス)
• [Int'l Joint Research] ボルドー大学(フランス)
• [Journal Article] Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation (2021)
  • Author(s): Mamode Cassim Adiilah、Navon Yotam、Gao Yu、Decossas Marion、Fouillen Laetitia、Grelard Axelle、Nagano Minoru、Lambert Olivier、Bahammou Delphine、Van Delft Pierre、Maneta-Peyret Lilly、Simon-Plas Francoise、Heux Laurent、Jean Bruno、Fragneto Giovanna、Mortimer Jenny C.、Deleu Magali、Lins Laurence、Mongrand Sebastien
  • Journal Title: Journal of Biological Chemistry Volume: - Pages: 100602-100602
  • DOI: 10.1016/j.jbc.2021.100602
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b5 -B (2020)
  • Author(s): Nagano Minoru、Ueda Haruko、Fukao Yoichiro、Kawai-Yamada Maki、Hara-Nishimura Ikuko
  • Journal Title: Plant Signaling & Behavior Volume: 15 Issue: 9 Pages: 1790196-1790196
  • DOI: 10.1080/15592324.2020.1790196
  • Peer Reviewed / Open Access
• [Journal Article] An Arabidopsis NAC domain transcription factor, ATAF2, promotes age‐dependent and dark‐induced leaf senescence (2020)
  • Author(s): Nagahage Isura Sumeda Priyadarshana、Sakamoto Shingo、Nagano Minoru、Ishikawa Toshiki、Mitsuda Nobutaka、Kawai‐Yamada Maki、Yamaguchi Masatoshi
  • Journal Title: Physiologia Plantarum Volume: 170 Issue: 2 Pages: 299-308
  • DOI: 10.1111/ppl.13156
  • Peer Reviewed / Open Access
• [Journal Article] Plant-Unique cis/trans Isomerism of Long-Chain Base Unsaturation is Selectively Required for Aluminum Tolerance Resulting from Glucosylceramide-Dependent Plasma Membrane Fluidity (2020)
  • Author(s): Sato Masaya、Nagano Minoru、Jin Song、Miyagi Atsuko、Yamaguchi Masatoshi、Kawai-Yamada Maki、Ishikawa Toshiki
  • Journal Title: Plants Volume: 9 Issue: 1 Pages: 19-19
  • DOI: 10.3390/plants9010019
  • Peer Reviewed / Open Access
• [Journal Article] Arabidopsis Bax inhibitor-1 interacts with enzymes related to very-long-chain fatty acid synthesis (2019)
  • Author(s): Nagano M., Kakuta C., Fukao Y., Fujiwara M., Uchimiya H., Kawai-Yamada M.
  • Journal Title: J. Plant Res. Volume: 132 Issue: 1 Pages: 131-143
  • DOI: 10.1007/s10265-018-01081-8
  • NAID: 40021808152
  • Peer Reviewed
• [Journal Article] One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 6803 (2019)
  • Author(s): Ishikawa Y., Miyagi A., Ishikawa T., Nagano M., Yamaguchi M., Hihara Y., Kaneko Y., Kawai-Yamada M.
  • Journal Title: Plant J. Volume: - Issue: 4 Pages: 654-666
  • DOI: 10.1111/tpj.14262
  • Peer Reviewed / Open Access
• [Presentation] 植物ナノドメインの機能解明を目指した可視化プローブの開発 (2020)
  • Author(s): 長野稔
  • Organizer: 日本植物学会第84回大会
  • Invited
• [Presentation] 植物スフィンゴ脂質が制御する病害抵抗性機構 (2020)
  • Author(s): 長野稔
  • Organizer: 第62回日本脂質生化学会
  • Invited
• [Presentation] 細胞膜を介した植物免疫制御機構の解析 (2019)
  • Author(s): 長野稔、宇川智水、伴野文彦、石川寿樹、山口雅利、深尾陽一朗、川合真紀
  • Organizer: 日本植物学会第83回大会
• [Presentation] スフィンゴ脂質2-ヒドロキシル化が制御するシロイヌナズナ免疫システムの解析 (2019)
  • Author(s): 長野稔、宇川智水、伴野文彦、石川寿樹、山口雅利、深尾陽一朗、川合真紀
  • Organizer: 第32回植物脂質シンポジウム
• [Presentation] Functional association of 2-hydroxy sphingolipids with innate immunity in Arabidopsis (2019)
  • Author(s): Nagano M, Ukawa T, Banno F, Ishikawa T, Yamaguchi M, Fukao Y, Kawai-Yamada M
  • Organizer: 8th Asian-Ocean Symposium on Plant Lipids
  • Int'l Joint Research
• [Presentation] The Role of Sphingolipids in the Dynamics of Plasma Membrane in Plants (2019)
  • Author(s): Nagano M., Boutte Y., Mamode-Cassim A., Fouillen L., Kawai-Yamada M., Mongrand S.
  • Organizer: Gordon Research Conference, Plant Lipids: Structure, Metabolism and Function
  • Int'l Joint Research
• [Presentation] 植物の細胞膜動態におけるスフィンゴ脂質の役割 (2019)
  • Author(s): 長野稔、Boutte Y., Mamode-Cassim A., Fouillen L., 川合真紀, Mongrand S.
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] スフィンゴ脂質による植物細胞膜ダイナミクスの制御 (2018)
  • Author(s): 長野稔、Boutte Y., Mamode-Cassim A., Fouillen L., 川合真紀, Mongrand S.
  • Organizer: 日本植物学会第82回大会
• [Presentation] The Role of Sphingolipids in the Dynamics of Plasma Membrane in Plants (2018)
  • Author(s): Nagano M., Boutte Y., Mamode-Cassim A., Fouillen L., Kawai-Yamada M., Mongrand S.
  • Organizer: The 23rd International Symposium on Plant Lipids
  • Int'l Joint Research
• [Presentation] Arabidopsis Bax inhibitor-1 promotes VLCFA synthesis through the interaction with VLCFA-related enzymes (2017)
  • Author(s): Minoru Nagano, Chikako Kakuta, Masayuki Fujiwara, Yoichiro Fukao and Maki Kawai-Yamada
  • Organizer: 28th International Conference on Arabidopsis Research
  • Int'l Joint Research
• [Presentation] 2-ヒドロキシスフィンゴ脂質によるシロイヌナズナ耐病性の解析 (2017)
  • Author(s): 宇川智水、長野稔、石川寿樹、山口雅利、川合真紀
  • Organizer: 日本植物学会第81回大会
• [Presentation] The role of plasma membrane microdomains in plant innate immunity (2017)
  • Author(s): Minoru Nagano and Maki Kawai-Yamada
  • Organizer: GDRI-IPB NETWORK
  • Int'l Joint Research
• [Presentation] Analysis of the impact of 2-hydroxy sphingolipids on disease resistance in Arabidopsis thaliana (2017)
  • Author(s): Tomomi Ukawa, Minoru Nagano, Toshiki Ishikawa, Masatoshi Yamaguchi and Maki Kawai-Yamada
  • Organizer: The 7th Asian Symposium on Plant Lipid
  • Int'l Joint Research
• [Remarks]
  • URL: https://fukaoyoichirolab.wixsite.com/rits